Somatosensory Neuron Typing with High-Coverage Single-Cell RNA Sequencing and Functional Analysis.
10.1007/s12264-017-0147-9
- Author:
Changlin LI
1
;
Sashuang WANG
1
;
Yan CHEN
1
;
Xu ZHANG
2
Author Information
1. Institute of Neuroscience and State Key Laboratory of Neuroscience, Center for Excellence in Brain Science, Chinese Academy of Sciences, Shanghai, 200031, China.
2. Institute of Neuroscience and State Key Laboratory of Neuroscience, Center for Excellence in Brain Science, Chinese Academy of Sciences, Shanghai, 200031, China. xu.zhang@ion.ac.cn.
- Publication Type:Journal Article
- Keywords:
Gene profiles;
Neuron type;
Pain;
Single-cell technology;
Somatosensory mechanism
- MeSH:
Animals;
Ganglia, Spinal;
cytology;
Gene Regulatory Networks;
Humans;
Pain;
genetics;
metabolism;
pathology;
Sensory Receptor Cells;
metabolism;
Sequence Analysis, RNA;
Transcriptome
- From:Neuroscience Bulletin
2018;34(1):200-207
- CountryChina
- Language:English
-
Abstract:
Different physical and chemical stimuli are detected by the peripheral sensory receptors of dorsal root ganglion (DRG) neurons, and the generated inputs are transmitted via afferent fibers into the central nervous system. The gene expression profiles of DRG neurons contribute to the generation, transmission, and regulation of various somatosensory signals. Recently, the single-cell transcriptomes, cell types, and functional annotations of somatosensory neurons have been studied. In this review, we introduce our classification of DRG neurons based on single-cell RNA-sequencing and functional analyses, and discuss the technical approaches. Moreover, studies on the molecular and cellular mechanisms underlying somatic sensations are discussed.
