Rat Cerebrospinal Fluid Treatment Method through Cisterna Cerebellomedullaris Injection.
10.1007/s12264-018-0223-9
- Author:
Thainá Garbino DOS SANTOS
1
;
Mery Stéfani Leivas PEREIRA
2
;
Diogo Losch OLIVEIRA
2
Author Information
1. Laboratory of Cellular Neurochemistry, Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, 90035-003, Brazil. thainagarbino@gmail.com.
2. Laboratory of Cellular Neurochemistry, Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, 90035-003, Brazil.
- Publication Type:Journal Article
- Keywords:
Central nervous system;
Cerebrospinal fluid treatment;
Cisterna magna;
Drug administration;
Intracisternal injection;
Neurobiology;
Neuroscience;
Rodent;
Surgical technique
- MeSH:
Anesthesia;
Animals;
Central Nervous System Agents;
administration & dosage;
Cerebrospinal Fluid;
Cisterna Magna;
Contrast Media;
Excitatory Amino Acid Agents;
administration & dosage;
Glioma;
drug therapy;
Methylene Blue;
Models, Animal;
Rats, Wistar
- From:
Neuroscience Bulletin
2018;34(5):827-832
- CountryChina
- Language:English
-
Abstract:
Drugs that lack the ability to cross the blood-brain barrier (BBB) need to be placed directly into the central nervous system. Our laboratory studies the involvement of the glutamatergic system in the aggressiveness of glioma, and some ligands of glutamate receptors cannot permeate the BBB. Here, glioma-implanted rats were treated by a technique that delivers ligands directly into the cerebrospinal fluid by puncture into the cisterna cerebellomedullaris. Rats were anesthetized and fixed in a rodent stereotactic device. The head was gently tilted downwards at an angle that allowed exposure of the cisterna. Injection into the cisterna was done freehand using a gingival needle coupled to a microsyringe. The efficiency of intracisternal injection was demonstrated using a methylene blue solution. This type of injection is adaptable for any rodent model using small volumes of a variety of other drugs, and is an interesting method for neuroscience studies.
