Transcriptomic analysis in Anemone flaccida rhizomes reveals ancillary pathway for triterpene saponins biosynthesis and differential responsiveness to phytohormones.
10.1016/S1875-5364(19)30015-9
- Author:
Guo-Yan MO
1
;
Fang HUANG
1
;
Yin FANG
1
;
Lin-Tao HAN
2
;
Kayla K PENNERMAN
3
;
Li-Jing BU
4
;
Xiao-Wei DU
5
;
Joan W BENNETT
3
;
Guo-Hua YIN
6
Author Information
1. China Key Laboratory of TCM Resource and Prescription, Ministry of Education, Hubei University of Chinese Medicine, Wuhan 430065, China.
2. China Key Laboratory of TCM Resource and Prescription, Ministry of Education, Hubei University of Chinese Medicine, Wuhan 430065, China. Electronic address: hanlt711.metabonomics@gmail.com.
3. Department of Plant Biology and Pathology, Rutgers, The State University of New Jersey, New Jersey 08901, USA.
4. Department of Biology, University of New Mexico, Albuquerque, New Mexico 87131, USA.
5. Department of Pharmacy, Heilongjiang University of Chinese Medicine, Harbin 150040, China.
6. Department of Plant Biology and Pathology, Rutgers, The State University of New Jersey, New Jersey 08901, USA. Electronic address: guohuayin1997@gmail.com.
- Publication Type:Journal Article
- Keywords:
Anemone flaccida Fr. Schmidt;
Biosynthetic pathways;
Phytohormonal responsiveness;
Transcriptomic analysis;
Triterpenoid saponins
- MeSH:
Anemone;
drug effects;
genetics;
metabolism;
Biosynthetic Pathways;
drug effects;
genetics;
Cytochrome P-450 Enzyme System;
genetics;
metabolism;
Gene Expression Profiling;
Gene Expression Regulation, Plant;
drug effects;
Glycosyltransferases;
genetics;
metabolism;
Oleanolic Acid;
analogs & derivatives;
metabolism;
Plant Growth Regulators;
pharmacology;
Plant Proteins;
genetics;
metabolism;
Plants, Medicinal;
Rhizome;
drug effects;
genetics;
metabolism;
Saponins;
metabolism;
Triterpenes;
metabolism
- From:
Chinese Journal of Natural Medicines (English Ed.)
2019;17(2):131-144
- CountryChina
- Language:English
-
Abstract:
Anemone flaccida Fr. Schmidt is a perennial medicinal herb that contains pentacyclic triterpenoid saponins as the major bioactive constituents. In China, the rhizomes are used as treatments for a variety of ailments including arthritis. However, yields of the saponins are low, and little is known about the plant's genetic background or phytohormonal responsiveness. Using one-quarter of the 454 pyrosequencing information from the Roche GS FLX Titanium platform, we performed a transcriptomic analysis to identify 157 genes putatively encoding 26 enzymes involved in the synthesis of the bioactive compounds. It was revealed that there are two biosynthetic pathways of triterpene saponins in A. flaccida. One pathway depends on β-amyrin synthase and is similar to that found in other plants. The second, subsidiary ("backburner") pathway is catalyzed by camelliol C synthase and yields β-amyrin as minor byproduct. Both pathways used cytochrome P450-dependent monooxygenases (CYPs) and family 1 uridine diphosphate glycosyltransferases (UGTs) to modify the triterpenoid backbone. The expression of CYPs and UGTs were quite different in roots treated with the phytohormones methyl jasmonate, salicylic acid and indole-3-acetic acid. This study provides the first large-scale transcriptional dataset for the biosynthetic pathways of triterpene saponins and their phytohormonal responsiveness in the genus Anemone.
