Dalestones A and B, two anti-inflammatory cyclopentenones from Daldinia eschscholzii with an edited strong promoter for the global regulator LaeA-like gene.
10.1016/S1875-5364(19)30045-7
- Author:
Zhen-Zhen ZHOU
1
;
Hong-Jie ZHU
1
;
Cheng-Long YANG
1
;
Yan-Jun LIU
2
;
Nan JIANG
3
;
Yong-Sheng XIAO
1
;
Li-Yun SHI
2
;
Rui-Hua JIAO
1
;
Hui-Ming GE
4
;
Ren-Xiang TAN
5
,
6
Author Information
1. State Key Laboratory of Pharmaceutical Biotechnology, Institute of Functional Biomolecules, Nanjing University, Nanjing 210023, China.
2. State Key Laboratory Cultivation Base for TCM Quality and Efficacy, Nanjing University of Chinese Medicine, Nanjing 210023, China.
3. School of Pharmacy, Nanjing Medical University, Nanjing, 210029, China.
4. State Key Laboratory of Pharmaceutical Biotechnology, Institute of Functional Biomolecules, Nanjing University, Nanjing 210023, China. Electronic address: hmge@nju.edu.cn.
5. State Key Laboratory of Pharmaceutical Biotechnology, Institute of Functional Biomolecules, Nanjing University, Nanjing 210023, China
6. State Key Laboratory Cultivation Base for TCM Quality and Efficacy, Nanjing University of Chinese Medicine, Nanjing 210023, China. Electronic address: rxtan@nju.edu.cn.
- Publication Type:Journal Article
- Keywords:
Cyclopentenone;
Daldinia eschscholzii;
Global regulator
- From:
Chinese Journal of Natural Medicines (English Ed.)
2019;17(5):387-393
- CountryChina
- Language:English
-
Abstract:
Replacement of the native promoter of theglobal regulator LaeA-like gene of Daldinia eschscholzii by a strong gpdA promoter led to the generation of two novel cyclopentenone metabolites, named dalestones A and B, whose structures were assigned by a combination of spectroscopic analysis, modified Mosher's reaction, and electronic circular dichroism (ECD). Dalestones A and B inhibit the gene expression of TNF-α and IL-6 in LPS-induced RAW264.7 macrophages.
