Pretreatment of Populus tomentiglandulosa protects hippocampal CA1 pyramidal neurons from ischemia-reperfusion injury in gerbils via increasing SODs expressions and maintaining BDNF and IGF-I expressions.
10.1016/S1875-5364(19)30050-0
- Author:
Tae-Kyeong LEE
1
;
Joon Ha PARK
2
;
Ji Hyeon AHN
2
;
Hyunjung KIM
1
;
Minah SONG
1
;
Jae-Chul LEE
1
;
Jong Dai KIM
3
;
Yong Hwan JEON
4
;
Jung Hoon CHOI
5
;
Choong Hyun LEE
6
;
In Koo HWANG
7
;
Bing-Chun YAN
8
;
Moo-Ho WON
9
;
Il Jun KANG
10
Author Information
1. Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon 24341, Republic of Korea.
2. Department of Biomedical Science, Research Institute of Bioscience and Biotechnology, Hallym University, Chuncheon 24252, Republic of Korea.
3. Division of Food Biotechnology, School of Biotechnology, Kangwon National University, Chuncheon 24341, Republic of Korea.
4. Department of Radiology, School of Medicine, Kangwon National University, Chuncheon 24289, Republic of Korea.
5. Department of Anatomy, College of Veterinary Medicine, Kangwon National University, Chuncheon 24341, Republic of Korea.
6. Department of Pharmacy, College of Pharmacy, Dankook University, Cheonan 31116, Republic of Korea.
7. Department of Anatomy and Cell Biology, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 08826, Republic of Korea.
8. Jiangsu Key Laboratory of Integrated Traditional Chinese, Western Medicine for Prevention and Treatment of Senile Diseases, Yangzhou 225001, China.
9. Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon 24341, Republic of Korea. Electronic address: mhwon@kangwon.ac.kr.
10. Department of Food Science and Nutrition, Hallym University, Chuncheon 24252, Republic of Korea. Electronic address: ijkang@hallym.ac.kr.
- Publication Type:Journal Article
- Keywords:
Antioxidant enzymes;
Neuroprotective effects;
Populus tomentiglandulosa;
Transient global cerebral ischemia
- MeSH:
Animals;
Brain-Derived Neurotrophic Factor;
genetics;
metabolism;
CA1 Region, Hippocampal;
drug effects;
metabolism;
Gerbillinae;
Humans;
Insulin-Like Growth Factor I;
genetics;
metabolism;
Male;
Neuroprotective Agents;
administration & dosage;
Plant Extracts;
administration & dosage;
Populus;
chemistry;
Pyramidal Cells;
drug effects;
metabolism;
Reperfusion Injury;
drug therapy;
genetics;
metabolism;
Superoxide Dismutase;
genetics;
metabolism;
Up-Regulation;
drug effects
- From:
Chinese Journal of Natural Medicines (English Ed.)
2019;17(6):424-434
- CountryChina
- Language:English
-
Abstract:
To examine the effects of Populus tomentiglandulosa (PT) extract on the expressions of antioxidant enzymes and neurotrophic factors in the cornu ammonis 1 (CA1) region of the hippocampus at 5 min after inducing transient global cerebral ischemia (TGCI) in gerbils, TGCI was induced by occlusion of common carotid arteries for 5 min. Before ischemic surgery, 200 mg·kg PT extract was orally administrated once daily for 7 d. We performed neuronal nuclear antigen immunohistochemistry and Fluoro-Jade B staining. Furthermore, we determined in situ production of superoxide anion radical, expression levels of SOD1 and SOD2 as antioxidant enzymes and brain-derived neurotrophic factor (BDNF) and insulin-like growth factor I (IGF-I) as neurotrophic factors. Pretreatment with 200 mg·kg PT extract prevented neuronal death (loss). Furthermore, pretreatment with 200 mg·kg PT extract significantly inhibited the production of superoxide anion radical, increased expressions of SODs and maintained expressions of BDNF and IGF-I. Such increased expressions of SODs were maintained in the neurons after IRI. In summary, pretreated PT extract can significantly increase levels of SODs and protect the neurons against TGCI, suggesting that PT can be a useful natural agent to protect against TGCI.
