Species identification of poisonous medicinal plant using DNA barcoding.
10.1016/S1875-5364(19)30060-3
- Author:
Miao LIU
1
,
2
;
Xi-Wen LI
3
;
Bao-Sheng LIAO
4
;
Lu LUO
4
;
Yue-Ying REN
5
Author Information
1. College of Chinese Medicine Materials, Jilin Agriculture University, Changchun 130118, China
2. Institute of Chinese Materia Medica, Academy of Chinese Medical Sciences, Beijing 100700, China.
3. Institute of Chinese Materia Medica, Academy of Chinese Medical Sciences, Beijing 100700, China. Electronic address: xwli@icmm.ac.cn.
4. Institute of Chinese Materia Medica, Academy of Chinese Medical Sciences, Beijing 100700, China.
5. College of Chinese Medicine Materials, Jilin Agriculture University, Changchun 130118, China. Electronic address: renyueying@yahoo.com.cn.
- Publication Type:Journal Article
- Keywords:
Blast;
DNA barcoding;
Genetic distance;
ITS2;
Poisonous herbal medicine;
Sequence
- From:
Chinese Journal of Natural Medicines (English Ed.)
2019;17(8):585-590
- CountryChina
- Language:English
-
Abstract:
The aim is to select a universal DNA barcode for identifying all poisonous medicinal plants in Chinese pharmacopoeia and their poisonous related species or adulterants. We chose 4 commonly used regions as candidate DNA barcodes (ITS2, psbA-trnH, matK and rbcL) and compared their identification efficiency in 106 species from 27 families and 65 genera totally. Data analysis was performed including the information of sequence alignment, inter/intra-specific genetic distance and data distribution, identification efficiency and the situation of Neighbor-Joining (NJ) phylogenetic trees. We found ITS2 sequence region had high variation, stable genetic distance and identification efficiency relatively. The topological structure of NJ phylogenetic tree showed monophyletic. Our findings show that ITS2 can be applied as a universal barcode for identifying poisonous medicinal plants in Chinese pharmacopoeia and their poisonous related species or adulterants.
