Catharmus tinctorius volatile oil promote the migration of mesenchymal stem cells via ROCK2/Myosin light chain signaling.
10.1016/S1875-5364(19)30072-X
- Author:
Ya-Mei LIU
1
,
2
;
Wang-Yang LI
3
;
Liang-Liang XU
4
;
Li-Juan YU
5
;
Yi-Wen LUO
6
;
Xi-Can LI
7
;
Xun-Chao ZHANG
6
;
Yun-Pu XIONG
8
;
Hong-Tai CHEN
6
;
Jun-Lang ZHU
6
;
Chen CHEN
1
,
2
;
Yu-Lu XIE
7
;
Dong-Feng CHEN
9
;
Bin WANG
10
Author Information
1. College of Basic Medical, Guangzhou University of Chinese Medicine, Guangzhou 510006, China
2. Innovative Research & Development Lab. of TCM, Guangzhou University of Chinese Medicine, Guangzhou 510006, China.
3. The Second Affiliated Hospital of Hunan University of Chinese Medicine, Changsha 410208, China.
4. The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Lingnan Medical Research Center of Guangzhou University of Chinese Medicine, Guangzhou 510000, China.
5. College of Basic Medical, Guangzhou University of Chinese Medicine, Guangzhou 510006, China.
6. Department of Traumatology, the Third Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine, Guangzhou 510240, China.
7. School of Chinese Herbal Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510006, China.
8. Department of Orthopaedics, Guangzhou Orthopedic hospital, Guangzhou 510030, China.
9. Department of Anatomy, The Research Center of Basic Integrative Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510006, China. Electronic address: df27212@21cn.com.
10. Department of Traumatology, the Third Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine, Guangzhou 510240, China. Electronic address: wangbin1973@163.com.
- Publication Type:Journal Article
- Keywords:
Catharmus tinctorius;
MSC;
Migration;
ROCK2/MLC signaling;
Volatile oil
- From:
Chinese Journal of Natural Medicines (English Ed.)
2019;17(7):506-516
- CountryChina
- Language:English
-
Abstract:
MSC transplantation has been explored as a new clinical approach to stem cell-based therapies for bone diseases in regenerative medicine due to their osteogenic capability. However, only a small population of implanted MSC could successfully reach the injured areas. Therefore, enhancing MSC migration could be a beneficial strategy to improve the therapeutic potential of cell transplantation. Catharmus tinctorius volatile oil (CTVO) was found to facilitate MSC migration. Further exploration of the underlying molecular mechanism participating in the pro-migratory ability may provide a novel strategy to improve MSC transplantation efficacy. This study indicated that CTVO promotes MSC migration through enhancing ROCK2 mRNA and protein expressions. MSC migration induced by CTVO was blunted by ROCK2 inhibitor, which also decreased myosin light chain (MLC) phosphorylation. Meanwhile, the siRNA for ROCK2 inhibited the effect of CTVO on MSC migration ability and attenuated MLC phosphorylation, suggesting that CTVO may promote BMSC migration via the ROCK2/MLC signaling. Taken together, this study indicates that C. tinctorius volatile oil could enhance MSC migration via ROCK2/MLC signaling in vitro. C. tinctorius volatile oil-targeted therapy could be a beneficial strategy to improve the therapeutic potential of cell transplantation for bone diseases in regenerative medicine.
