Steroidal constituents from Helleborus thibetanus and their cytotoxicities.
10.1016/S1875-5364(19)30094-9
- Author:
Yu-Ze LI
1
;
Hua-Wei ZHANG
2
;
Hao FAN
2
;
Xiao-Fei LIANG
2
;
Bei SONG
1
;
Huan CHEN
1
;
Wen-Li HUANG
2
;
Zheng-Gang YUE
2
;
Xiao-Mei SONG
3
;
Jian-Li LIU
4
Author Information
1. Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, College of Life Sciences, Northwest University, Xi'an 710069, China.
2. Shaanxi Collaborative Innovation Center of Chinese Medicinal Resource Industrialization, School of Pharmacy, Shaanxi University of Chinese Medicine, Xianyang 712046, China.
3. Shaanxi Collaborative Innovation Center of Chinese Medicinal Resource Industrialization, School of Pharmacy, Shaanxi University of Chinese Medicine, Xianyang 712046, China. Electronic address: Songxiaom@126.com.
4. Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, College of Life Sciences, Northwest University, Xi'an 710069, China. Electronic address: jlliu@nwu.edu.cn.
- Publication Type:Journal Article
- Keywords:
Cytotoxic activity;
Helleborus thibetanus;
Steroidal constituents;
Structure identification
- From:
Chinese Journal of Natural Medicines (English Ed.)
2019;17(10):778-784
- CountryChina
- Language:English
-
Abstract:
Thibetanosides E-H (1-4), four new steroidal constituents including three rare sulfonates (2-4), were isolated from the roots and rhizomes of Helleborus thibetanus, together with nine known steroidal compounds (5-13). Their structures were elucidated by detailed spectroscopic analysis, including 1D and 2D NMR techniques and chemical evidence. In this study, compounds 2-13 were evaluated for their cytotoxic activities against HCT116, A549 and HepG2 tumor cell lines in vitro. Among them, compound 8 (thibetanoside C) showed cytotoxicities against A549 cells(IC 39.6 ± 1.9 μmol·L) and HepG2 cells(IC 41.5 ± 1.1 μmol·L), respectively. Compound 9 (23S, 24S)-24-[(O-β-D-fucopyranosyl)oxy]-3β, 23-dihydroxy-spirosta-5, 25(27)-diene-1β-ylO-(4-O-acetyl- α-L-rhamnopyranosyl)-(1→2)-O-[β-D-xylopyranosyl-(1→3)]-α-L-arabinopyranoside) showed cytotoxicity against HCT116 cells(IC 33.6 ± 2.1 μmol·L).
