Purification, structure characterization and antioxidant activity of polysaccharides from Saposhnikovia divaricata.
10.1016/S1875-5364(19)30096-2
- Author:
Yao MENG
1
;
Lin YI
1
;
Lei CHEN
1
;
Jie HAO
1
;
Du-Xin LI
1
;
Jie XUE
1
;
Nai-Yu XU
2
;
Zhen-Qing ZHANG
3
,
4
Author Information
1. College of Pharmaceutical Sciences, Soochow University, Suzhou 215123, China.
2. Jiangsu Key Laboratory of Translational Research and Therapy for Neuro-Psycho-Diseases, Soochow University, Suzhou 215021, China.
3. College of Pharmaceutical Sciences, Soochow University, Suzhou 215123, China
4. Jiangsu Key Laboratory of Translational Research and Therapy for Neuro-Psycho-Diseases, Soochow University, Suzhou 215021, China. Electronic address: z_zhang@suda.edu.cn.
- Publication Type:Journal Article
- Keywords:
Antioxidant activity;
Arabinogalactan;
Polysaccharides;
Saposhnikovia divaricata
- From:
Chinese Journal of Natural Medicines (English Ed.)
2019;17(10):792-800
- CountryChina
- Language:English
-
Abstract:
Polysaccharide from traditional Chinese herb, Saposhnikovia divaricata (Turcz.) Schischk. (SD) was extracted, fractionated and characterized in this work. Four fractions were prepared. Their molecular weight, monosaccharide compositions, linkage modes and structural properties were characterized with SEC-MALS-RI, HPAEC-PAD, GC-MS and NMR. SDP1 was assigned as a 1, 4-α-glucan with small amount of O-6 linked branches. SDP2 contained a big amount of the 1, 4-α-glucan and a small amount of arabinogalactan, while SDP3 possessed relatively lower amount of the 1, 4-α-glucan and a big amount of the arabinogalactan. SDP4 was defined as a pectic arabinogalactan. Four fractions showed antioxidant activities in both molecular and cellular levels and their activity was ranked as SDP4 ≈ SDP3>SDP2>SDP1. The 1, 4-α-glucan in SDP1 had the weakest, while SDP3 and SDP4 showed similar and the highest antioxidant activity. The arabinogalactan was the major component of both SDP3 and SDP4, which significantly contributed to the antioxidant activity of SDP.
