Expression profiles of microRNA in peripheral blood mononuclear cells and immune factors in pregnant women with hepatitis B virus infection.
10.3760/cma.j.issn.1003-9406.2019.08.007
- Author:
Yun WANG
1
;
Xia ZHANG
;
Qingmiao YU
;
Fan JIN
;
Xiaofeng JIANG
Author Information
1. Department of Reproductive Endocrinology, Women' s Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310006, China. jinfan@zju.edu.cn.
- Publication Type:Journal Article
- MeSH:
Cytokines;
blood;
DNA, Viral;
Female;
Hepatitis B;
blood;
Hepatitis B virus;
Humans;
Leukocytes, Mononuclear;
metabolism;
MicroRNAs;
blood;
Pregnancy
- From:
Chinese Journal of Medical Genetics
2019;36(8):781-784
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To determine the expression profile of microRNA (miRNA) in peripheral blood mononuclear cells (PBMC) and immune factors in pregnant women with hepatitis B virus (HBV) infection.
METHODS:A total of 182 pregnant women infected with HBV were randomly selected, with 40 healthy pregnant women and 35 non-pregnant women as controls. High-throughput sequencing was used to detect RNA in the PBMC of all subjects. Indirect ELISA method was used to determine the changes of cytokines in peripheral blood samples.
RESULTS:Compared with the control group, 18 differentially expressed miRNA were identified in those with HBV infection (P< 0.01). Among these, miR-3607-3p, miR-20a, miR-1296, miR-153-1 and miR-X4 may directly regulate the transcriptional level of target genes including IL-10, IL-18, IL-16, MCP-1, NUP50 and CCR1. Meanwhile, peripheral blood cytokines IL-10, IL-18, IL-16 and MCP-1 were significantly increased in those with HBV infection (P<0.01), with the expression level of IL-16 and MCP-1 being strongly correlated with the viral load.
CONCLUSION:The expression profiles of miRNA in PBMC and cytokines in peripheral blood can change significantly during pregnancy, both may be involved in the immune response to HBV infection.
