Effect of miR-137 on the proliferation and apoptosis of endothelial cells regulated by AKT2 gene.
10.3760/cma.j.issn.1003-9406.2019.10.006
- VernacularTitle:miR-137调节AKT2对内皮细胞增殖与凋亡的影响
- Author:
Zemin XU
1
;
Yong LIU
;
Na SHEN
;
Tao FANG
;
Fengshi TIAN
Author Information
1. Fourth Center Clinical College of Tianjin Medical University, Tianjin Fourth Central Hospital, Internal Department of Cardiovascular Medicine, Tianjin 300140, China. fengshitian0801011@126.com.
- Publication Type:Journal Article
- MeSH:
Apoptosis;
Cell Proliferation;
Cells, Cultured;
Glucose;
Human Umbilical Vein Endothelial Cells;
cytology;
Humans;
MicroRNAs;
genetics;
Proto-Oncogene Proteins c-akt;
genetics
- From:
Chinese Journal of Medical Genetics
2019;36(10):975-979
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To assess the effect of miR-137 on the proliferation and apoptosis of human umbilical vein endothelial cells (HUVECs) induced by high glucose and its mechanism.
METHODS:HUVECs cells were divided into low-glucose group (5.5 mmol/L glucose-treated cells), high-glucose group (33.36 mmol/L glucose-treated cells), anti-NC group (cells treated with 33.36 mmol/L glucose after anti-NC transfection) and anti-miR-137 group (cells treated with 33.36 mmol/L glucose after anti-miR-137 transfection). After 48 hours, qRT-PCR was used to determine the expression of miR-137. CCK-8 assay and flow cytometry were used to detect cell proliferation and apoptosis rate, respectively. The targeting relationship between miR-137 and AKT2 was validated by dual fluorescence reporter gene detection system and AKT2 protein expression after overexpression or inhibition of miR-137.
RESULTS:High glucose could significantly up-regulate the expression of miR-137 in HUVECs cells, and the expression of miR-137 in HUVECs cells transfected with miR-137 inhibitor was significantly decreased (P<0.05). High glucose can significantly inhibit HUVECs cell proliferation and induce apoptosis, while inhibition of miR-137 expression can weaken the effect of high glucose on HUVECs cell proliferation inhibition and apoptosis promotion (P<0.05). Inhibiting AKT2 expression could weaken the inhibitory effect of miR-137 inhibitor on HUVECs cell proliferation and apoptosis (P<0.05).
CONCLUSION:Inhibiting the expression of miR-137 gene can attenuate the proliferation inhibition and apoptosis promotion of HUVECs induced by high glucose, and the mechanism is related to activating the expression of AKT2.
