Expression and significance of tight junction proteins in the kidney in a mouse model of renal ischemia-reperfusion injury.
- Author:
Zhi-Hui LI
1
;
Tuan-Hong XIA
;
Zhi-Juan KANG
;
Xu DENG
;
Ying WANG
Author Information
1. Department of Nephrology, Hunan Children's Hospital/Academy of Pediatrics of University of South China, Changsha 410007, China. Lizh0731@aliyun.com.
- Publication Type:Journal Article
- MeSH:
Animals;
Kidney;
Male;
Mice;
Mice, Inbred C57BL;
Rats, Sprague-Dawley;
Reperfusion Injury;
Tight Junction Proteins
- From:
Chinese Journal of Contemporary Pediatrics
2018;20(12):1055-1062
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the expression and significance of tight junction proteins (claudin-2, claudin-10, and claudin-17) in a mouse model of renal ischemia-reperfusion injury.
METHODS:A total of 152 male C57BL/6 mice were randomly assigned to control group (n=8), sham-operation group (n=72), and model group (n=72). The renal pedicles at both sides were clamped for 30 minutes to establish a mouse model of renal ischemia-reperfusion injury. According to the time points of reperfusion (0, 3, 6, 12, 24, 48, and 72 hours and 5 and 7 days), the sham-operation group and the model group were further divided into 9 subgroups, with 8 mice in each subgroup. RT-PCR and immunohistochemistry were used to measure the mRNA and protein expression of claudin-2, claudin-10, and claudin-17 in renal tissue.
RESULTS:The control and sham-operation groups had no significant changes in the mRNA and protein expression of claudin-2, claudin-10, and claudin-17 in renal tissue over the time of reperfusion (P>0.05). Compared with the control and sham-operation groups, the model group had decreased mRNA and protein expression of claudin-2 and claudin-10 after reperfusion, and the expression decreased gradually over the time of reperfusion, with the lowest levels at 24 hours of reperfusion (P<0.05). Compared with the control and sham-operation groups, the model group had increased mRNA and protein expression of claudin-17 after reperfusion, and the expression increased gradually over the time of reperfusion, with the highest mRNA level at 12 hours and the highest protein level at 24 hours of reperfusion (P<0.05).
CONCLUSIONS:Renal ischemia-reperfusion injury is closely associated with abnormal expression of tight junction proteins claudin-2, claudin-10, and claudin-17.
