Clinical value of droplet digital PCR in rapid diagnosis of invasive fungal infection in neonates.
- Author:
Hui-Tao LI
1
;
Bing-Chun LIN
;
Zhi-Feng HUANG
;
Chuan-Zhong YANG
;
Wei-Min HUANG
Author Information
1. Department of Neonatology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. hwmnet@21cn.com.
- Publication Type:Journal Article
- MeSH:
China;
Humans;
Infant, Newborn;
Invasive Fungal Infections;
Polymerase Chain Reaction;
Reproducibility of Results
- From:
Chinese Journal of Contemporary Pediatrics
2019;21(1):45-51
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To evaluate the clinical value of droplet digital PCR (ddPCR) in rapid and accurate diagnosis of invasive fungal infection (IFI) in neonates.
METHODS:The highly conserved sequence of fungi 18S RNA was selected as the target sequence, and primers were designed to establish a ddPCR fungal detection system. Blood samples were collected from 83 neonates with high-risk factors for IFI and/or related clinical symptoms in the neonatal intensive care unit (NICU) of a hospital in Shenzhen, China. Blood culture and ddPCR were used for fungal detection.
RESULTS:The ddPCR fungal detection system had a specificity of 100% and a sensitivity of 3.2 copies/μL, and had a good reproducibility. Among the 22 blood samples from neonates with a confirmed or clinical diagnosis of IFI, 19 were detected positive by ddPCR. Among the 61 blood samples from neonates who were suspected of IFI or had no IFI, 2 were detected positive by ddPCR.
CONCLUSIONS:The ddPCR technique can be used for the detection of neonatal IFI and is a promising tool for the screening and even diagnosis of neonatal IFI.
