Article Effect and Mechanism of Ganoderma lucidum Polysaccharides on Human Fibroblasts and Skin Wound Healing in Mice.
10.1007/s11655-018-3060-9
- Author:
Feng HU
1
;
Yu YAN
2
;
Chu-Wang WANG
1
;
Yu LIU
1
;
Jing-Jing WANG
2
;
Fang ZHOU
2
;
Qing-Hai ZENG
1
;
Xiao ZHOU
3
;
Jia CHEN
1
;
Ai-Jun WANG
4
;
Jian-da ZHOU
5
Author Information
1. Department of Plastic Surgery, the Third Xiangya Hospital of Central South University, Changsha, 410013, China.
2. Xiangya School of Medicine, Central South University, Changsha, 410013, China.
3. Department of Head and Neck Surgery, Hunan Province Cancer Hospital, Changsha, 410013, China.
4. Department of Surgery, Davis Health System, University of California, Sacramento, CA, 95817, USA.
5. Department of Plastic Surgery, the Third Xiangya Hospital of Central South University, Changsha, 410013, China. zhoujianda@csu.edu.cn.
- Publication Type:Journal Article
- Keywords:
Ganoderma lucidum polysaccharides;
Wnt/β-catenin signaling pathway;
human fibroblast;
transforming growth factor-β1;
wound healing
- MeSH:
Animals;
Cell Movement;
drug effects;
Cell Survival;
drug effects;
Cells, Cultured;
Collagen Type I;
biosynthesis;
Fibroblasts;
drug effects;
Humans;
Male;
Mice;
Polysaccharides;
pharmacology;
Reishi;
chemistry;
Skin;
drug effects;
injuries;
Transforming Growth Factor beta1;
physiology;
Wound Healing;
drug effects;
beta Catenin;
physiology
- From:
Chinese journal of integrative medicine
2019;25(3):203-209
- CountryChina
- Language:English
-
Abstract:
OBJECTIVE:To investigate the effects of Ganoderma lucidum polysaccharides (GL-PS) on human fibroblasts and skin wound healing in Kunming male mice and to explore the putative molecular mechanism.
METHODS:Primary human skin fibroblasts were cultured. The viability of fibroblasts treated with 0, 10, 20, 40, 80, and 160 μg/mL of GL-PS, respectively were detected by 3-4,5-dimethyl-2-thiazolyl-2,5-diphenyl-2-Htetrazolium bromide (MTT). The migration ability of fibroblasts treated with 0, 10, 20, and 40 μg/mL of GL-PS were measured by transwell assay. The secretion of the C-terminal peptide of procollagen type I (CICP) and transforming growth factor-β1 (TGF-β1) in the cell supernatant was tested by enzyme-linked immunosorbent assay. The expression of β-catenin was detected by Western blot. Furthermore, the Kunming mouse model with full-layer skin resection trauma was established, and was treated with 10, 20, and 40 mg/mL of GL-PS, respectively as external use. The size of the wound was measured daily, complete healing time in each group was recorded and the percentage of wound contraction was calculated.
RESULTS:Compared with the control group, 10, 20, and 40 μg/mL of GL-PS significantly increased the viability of fibroblasts, promoted the migration ability of fibroblasts, and up-regulated the expressions of CICP and TGF-β1 in fibroblasts (Plt;0.05 or Plt;0.01). The expression of β-catenin in fibroblasts treated with 20 and 40 μg/mL of GL-PS was significantly higher than that of the control group (Plt;0.01). Furthermore, after external use of 10, 20, and 40 mg/mL of GL-PS, the rates of wound healing in mice were significantly higher and the wound healing time was significantly less than the control group (Plt;0.05 or Plt;0.01).
CONCLUSION:A certain concentration of GL-PS may promote wound healing via activation of the Wnt/β-catenin signaling pathway and up-regulation of TGF-β1, which might serve as a promising source of skin wound healing.
