Protective effects of Shenmai injection on intestinal mucosal barrier function in severely scalded rats.
10.12047/j.cjap.5749.2019.012
- Author:
Chen YANG
1
;
Yao-Yao SONG
2
;
Yi-Fa JI
3
Author Information
1. Department of Burn and Plastic Surgery, Special Medical Center of Chinese People's Armed Police Force, Tianjin 300162.
2. Department of Burn and Plastic Surgery, the Sixth Medical Center of PLA General Hospital, Beijing 100048.
3. Department of Cosmetic Surgery, Beijing Fengtai Youanmen Hospital, Beijing 100069, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Bacterial Translocation;
drug effects;
Burns;
complications;
Drug Combinations;
Drugs, Chinese Herbal;
pharmacology;
Intestinal Mucosa;
drug effects;
pathology;
Random Allocation;
Rats;
Rats, Wistar
- From:
Chinese Journal of Applied Physiology
2019;35(1):47-49
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the protective effects of shen-mai injection on intestinal barrier function in the early stage of 30% 3° scald, and to provide experimental basis for the prevention and control of enterogenic infection.
METHODS:A total of 60 Wistar rats were randomly divided into 6 groups: normal control group (without treatment), model control group (with 30% total body surface area (TBSA) fully thickness burn on the back), hexadecadrol (5 mg/kg) group, Shenmai injection (5, 10, 15 mg/kg) groups, with 10 rats in each group. After burned by scald apparatus, rats in each group were treated with drugs immediately by intraperitoneal injection once a day. At 72 hours after burned, the levels of plasma endotoxin, diamine oxidase (DAO), tumor necrosis factor-alpha (TNF-α), interleukins-6(IL-6) in all rats were detected and the mesenteric lymph nodes, liver and spleen were homogenized to culture for bacteria. The change of secretory immunoglobulin A (sIgA) in intestinal mucosa was measured.
RESULTS:Compared with normal control group, bacterial translocation quantity in mesenteric lymph nodes(MLN), liver, and spleen, and the plasma levels of DAO, endotoxin, TNF-α, IL-6 and the level of sIgA in intestinal mucosa in model control group were increased significantly (P＜0.01); compared with model control group, bacterial translocation quantity in MLN, liver, and spleen, and the plasma levels of DAO, endotoxin, TNF-α, IL-6 and the level of sIgA in intestinal mucosa in hexadecadrol (5 mg/kg) group and shen-mai injection (5, 10, 15 mg/kg) groups were decreased significantly (P＜0.05 or P＜0.01).
CONCLUSION:Shen-mai injection can alleviate intestinal mucosa injury caused by severe scald, and the effects are similar with those of dexamethasone, and the effect is better in the high-dose group.
