Effects of Notch signal on the expressions of HIF-α and autophagy- related genes Beclin1, LC3I, LC3II in oxygen-glucose deprivation induced myocardial cell injury.
10.12047/j.cjap.5729.2019.036
- Author:
Ling Yu KONG
1
;
Zan XI
2
;
Wen Ting MA
3
;
Fei Yun YANG
1
;
Li Dan NIU
1
;
Jin He SHI
1
Author Information
1. Department of Emergency, the First Affiliated Hospital of Xinxiang Medical University, Weihui 453100.
2. Department of Pediatric Surgery, the First Affiliated Hospital of Xinxiang Medical University, Weihui 453100.
3. Department of Diagnostic Laboratory of Sanquan College, Xinxiang Medical University, Xinxiang 453003, China.
- Publication Type:Journal Article
- MeSH:
Autophagy;
Beclin-1;
metabolism;
Cell Hypoxia;
Cells, Cultured;
Glucose;
Humans;
Hypoxia-Inducible Factor 1, alpha Subunit;
metabolism;
Microtubule-Associated Proteins;
metabolism;
Myocytes, Cardiac;
cytology;
pathology;
Oxygen;
Receptors, Notch;
metabolism;
Signal Transduction
- From:
Chinese Journal of Applied Physiology
2019;35(2):165-168
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effects of Notch signal on hypoxic induction factor (HIF-1α) and autophagy-associated genes Beclin1, LC3I, LC3II in oxygen-glucose deprivation (OGD) induced myocardial cell injury.
METHODS:The OGD model was established using hypoxic culture box and hypoglycemic DMEM medium. The cells were divided into normal control group, OGD group, OGD + NC siRNA group, OGD + Notch1 siRNA group and OGD + HIF-1α siRNA group. Western blot was used to detect the interference effects of HIF-1α siRNA and Notch1 siRNA. The effects of Notch1 siRNA and HIF-1α siRNA on the activity of myocardial cells in OGD model were detected by the CCK-8 assay. The effects of Notch1 siRNA and HIF-1α siRNA on autophage-associated genes Beclin1, LC3I and LC3II expression were detected by Western blot.
RESULTS:The results of Western blot showed that HIF-1α siRNA could effectively knock down the expression of HIF-1α in myocardial cells in OGD model, and Notch1 siRNA could effectively knock down the expression of Notch1 and HIF-1α in myocardial cells in OGD model. The result of CCK-8 assay showed that Notch1 siRNA and HIF-1α siRNA reduced the activity of myocardial cells in OGD model, and there was no statistical difference between the two groups. Western blot results showed that Notch1 siRNA and HIF-1α siRNA could reduce the expressions of the autophagy-associated genes Beclin1, LC3I and LC3II, and reduce the ratio of LC3II to LC3I at mRNA level.
CONCLUSION:Notch1 plays a role in myocardial protection by regulating the expression of HIF-1α to regulate the autophagy in OGD model cells.
