Effects of apple polyphenols on monocrotaline-induced pulmonary vascular remodeling in rats and its mechanism.
10.12047/j.cjap.5769.2019.045
- Author:
Shu-Hao ZHANG
1
;
Si-Ming SHAO
2
;
Fang-Zheng CHEN
1
;
Jing ZHU
1
;
Luo-Wei CHEN
1
;
Heng WANG
3
;
Xin-Hui XIANG
2
;
Lin-Bo YUAN
4
Author Information
1. School of the First Clinical Medical Sciences, Wenzhou Medical University.
2. Ren ji College of Wenzhou Medical University.
3. College of Optometry, Wenzhou Medical University.
4. Department of Physiology, School of Basic Medical Sciences, Wenzhou Medical University, Wenzhou 325000, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Calcium;
metabolism;
Cyclooxygenase 2;
metabolism;
Cytokines;
metabolism;
Malus;
chemistry;
Monocrotaline;
Nitric Oxide;
metabolism;
Nitric Oxide Synthase Type III;
metabolism;
Polyphenols;
pharmacology;
Pulmonary Artery;
drug effects;
pathology;
Random Allocation;
Rats;
Vascular Remodeling;
drug effects
- From:
Chinese Journal of Applied Physiology
2019;35(3):209-214
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effects of apple polyphenols on pulmonary vascular remodeling in rats with pulmonary arterial hypertension and its mechanism.
METHODS:Rats were randomly divided into 4 groups:control (Con) group, monocrotaline (MCT) group, apple polyphenol (APP) group,monocrotaline + apple polyphenol (MCT+APP) group. In Con group, rats received a subcutaneous injection of physical saline. In APP group, rats received intraperitoneal injection of 20 mg/kg APP, every other day. In MCT group, rats received a single subcutaneous injection of MCT(60 mg/kg). In MCT+APP group, rats received subcutaneous injection of 60 mg/kg MCT followed by an intraperitoneal injection of 20 mg/kg APP every other day. All the disposal lasted 3 weeks. Then the PAH-relevant indicators, such as mean pulmonary artery pressure(mPAP), pulmonary vascular resistance(PVR), right ventricular hypertrophy index (RVHI) ,wall thickness (WT%) and wall area (WA%) were tested. After that, the inflammatory pathway related indicators, such as interleukin1(IL-1),interleukin1(IL-6), tumor necrosis factor α(TNF-α), cyclooxygenase 2(COX-2) and myeloperoxidase(MPO) in pulmonary tissue and free intracellular Ca in pulmonary smooth muscle cell(PASMC), content of eNOS and NO in endothelial cells were determined.
RESULTS:Compared with the control group, the levels of mPAP, PVR, RVHI, WA%, WT%, and IL-1, IL-6, TNF-α, COX-2, MPO in tissue and the expression of Ca in PASMC of MCT group were increased significantly, while the contents of eNOS and NO in endothelial cells were decreased significantly (P＜0.05). Compared with the MCT group, the apple polyphenol treatment could improve the above mentioned situation, and the COX-2 and Ca indicators of the apple polyphenol treatment group were decreased significantly (P＜0.05).
CONCLUSION:MCT can increase COX-2 expression and intracellular Ca in pulmonary artery smooth muscle cells, decrease the contents of eNOS and NO in endothelial cells, while apple polyphenols can significantly inhibit these effects.
