The effects of tectochrysin on prostate cancer cells apoptosis and its mechanism.
10.12047/j.cjap.5754.2019.059
- Author:
Yu WANG
1
;
Rui-Jun KE
1
;
Pan-Ruo JIANG
;
Jia-Hao YING
1
;
En-Zhe LOU
1
;
Jia-Yu CHEN
1
Author Information
1. Shaoxing University School of Medicine, Shaoxing 312000, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Apoptosis;
Cell Line, Tumor;
Flavonoids;
pharmacology;
Humans;
Male;
Mice;
Mice, Inbred BALB C;
Prostatic Neoplasms;
drug therapy;
pathology;
Signal Transduction;
TNF-Related Apoptosis-Inducing Ligand;
metabolism
- From:
Chinese Journal of Applied Physiology
2019;35(3):283-288
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effects of tectochrysin on prostate cancer cell line 22Rv.1 and reveal its molecular mechanism.
METHODS:Tectochrysin at the concentrations of 0～20 μg/ml was applied to 22Rv.1 cells and normal prostate cell RWPE-1. The proliferation activity of the cells was detected by MTS assay. Flow cytometry and hoechst 33342 staining were used to analyze the effects of drugs on cell apoptosis, death, cell cycle and nuclear type changes. LDH release test was used to analyze the cytotoxicity of the drug to 22Rv.1 cells. QPCR and Western blot were used to analyze the effects of the drug on the expressions of genes in 22Rv.1 cells. Finally, the tumor inhibited effect of the drug on the bearing tumor BALB/c mice were confirmed though anti-tumor experiment.
RESULTS:Tectochrysin could significantly inhibit the proliferation activity of 22Rv.1 cells and induced their apoptosis, and promoted the expressions of genes dr4, dr5, trail, p53, caspase-3, caspase-8, caspase-9, bid, bax and foxo3, inhibited the expressions of anti-apoptotic genes akt, pi3k and bcl-2.
CONCLUSION:Tectochrysin can induce prostate cancer cells apoptosis through affecting TRAIL and PI3K/AKT signaling pathways, and has anti-prostate cancer effect.
