Rapid and Sparse Labeling of Neurons Based on the Mutant Virus-Like Particle of Semliki Forest Virus.
10.1007/s12264-019-00362-z
- Author:
Fan JIA
1
;
Xutao ZHU
2
;
Pei LV
2
;
Liang HU
2
;
Qing LIU
3
;
Sen JIN
4
;
Fuqiang XU
5
Author Information
1. State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Key Laboratory of Magnetic Resonance in Biological Systems, Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences, Wuhan, 430071, China. jiafan@wipm.ac.cn.
2. Brain Research Center, Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences, Wuhan, 430071, China.
3. State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Key Laboratory of Magnetic Resonance in Biological Systems, Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences, Wuhan, 430071, China.
4. University of the Chinese Academy of Sciences, Beijing, 100049, China.
5. State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Key Laboratory of Magnetic Resonance in Biological Systems, Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences, Wuhan, 430071, China. fuqiang.xu@wipm.ac.cn.
- Publication Type:Journal Article
- Keywords:
Mutant virus-like particle;
Neuronal morphology;
Rapid labeling;
Semliki Forest virus;
Sparse labeling
- MeSH:
Animals;
Cells, Cultured;
Gene Expression;
Genetic Vectors;
genetics;
metabolism;
Green Fluorescent Proteins;
genetics;
metabolism;
Immunohistochemistry;
methods;
Male;
Mice, Inbred C57BL;
Microscopy, Fluorescence;
methods;
Neurons;
cytology;
metabolism;
Purkinje Cells;
cytology;
metabolism;
Semliki forest virus;
genetics
- From:
Neuroscience Bulletin
2019;35(3):378-388
- CountryChina
- Language:English
-
Abstract:
Sparse labeling of neurons contributes to uncovering their morphology, and rapid expression of a fluorescent protein reduces the experiment range. To achieve the goal of rapid and sparse labeling of neurons in vivo, we established a rapid method for depicting the fine structure of neurons at 24 h post-infection based on a mutant virus-like particle of Semliki Forest virus. Approximately 0.014 fluorescent focus-forming units of the mutant virus-like particle transferred enhanced green fluorescent protein into neurons in vivo, and its affinity for neurons in vivo was stronger than for neurons in vitro and BHK21 (baby hamster kidney) cells. Collectively, the mutant virus-like particle provides a robust and convenient way to reveal the fine structure of neurons and is expected to be a helper virus for combining with other tools to determine their connectivity. Our work adds a new tool to the approaches for rapid and sparse labeling of neurons in vivo.
