Brain-wide Mapping of Mono-synaptic Afferents to Different Cell Types in the Laterodorsal Tegmentum.
10.1007/s12264-019-00397-2
- Author:
Xiaomeng WANG
1
;
Hongbin YANG
1
;
Libiao PAN
1
;
Sijia HAO
1
;
Xiaotong WU
1
;
Li ZHAN
1
;
Yijun LIU
1
;
Fan MENG
1
;
Huifang LOU
1
;
Ying SHEN
1
;
Shumin DUAN
1
;
Hao WANG
2
Author Information
1. Department of Neurobiology and Department of Neurosurgery of The Second Affiliated Hospital, Key Laboratory of Medical Neurobiology of the Ministry of Health of China, NHC and CAMS Key Laboratory of Medical Neurobiology, Zhejiang University School of Medicine, Hangzhou, 310058, China.
2. Department of Neurobiology and Department of Neurosurgery of The Second Affiliated Hospital, Key Laboratory of Medical Neurobiology of the Ministry of Health of China, NHC and CAMS Key Laboratory of Medical Neurobiology, Zhejiang University School of Medicine, Hangzhou, 310058, China. haowang@zju.edu.cn.
- Publication Type:Journal Article
- Keywords:
Laterodorsal tegmentum;
Limbic lobe;
Mice;
Rabies virus retrograde tracing
- From:Neuroscience Bulletin
2019;35(5):781-790
- CountryChina
- Language:English
-
Abstract:
The laterodorsal tegmentum (LDT) is a brain structure involved in distinct behaviors including arousal, reward, and innate fear. How environmental stimuli and top-down control from high-order sensory and limbic cortical areas converge and coordinate in this region to modulate diverse behavioral outputs remains unclear. Using a modified rabies virus, we applied monosynaptic retrograde tracing to the whole brain to examine the LDT cell type specific upstream nuclei. The LDT received very strong midbrain and hindbrain afferents and moderate cortical and hypothalamic innervation but weak connections to the thalamus. The main projection neurons from cortical areas were restricted to the limbic lobe, including the ventral orbital cortex (VO), prelimbic, and cingulate cortices. Although different cell populations received qualitatively similar inputs, primarily via afferents from the periaqueductal gray area, superior colliculus, and the LDT itself, parvalbumin-positive (PV) GABAergic cells received preferential projections from local LDT neurons. With regard to the different subtypes of GABAergic cells, a considerable number of nuclei, including those of the ventral tegmental area, central amygdaloid nucleus, and VO, made significantly greater inputs to somatostatin-positive cells than to PV cells. Diverse inputs to the LDT on a system-wide level were revealed.
