Cloning and expression analysis of and quantification of downstream products in under flooding stress.
10.19540/j.cnki.cjcmm.20171030.008
- Author:
Qing-Jun ZOU
1
;
Tao WANG
1
;
Qiao-Sheng GUO
1
;
You-Mei XIAO
1
;
Li-Wei WU
1
Author Information
1. Institute of Chinese Medicinal Materials, Nanjing Agricultural University, Nanjing 210095, China.
- Publication Type:Journal Article
- Keywords:
Chrysanthemum morifolium;
F3'H;
active ingredients;
clone;
flavonoids;
flooding stress
- MeSH:
Chrysanthemum;
enzymology;
genetics;
Cloning, Molecular;
Cytochrome P-450 Enzyme System;
genetics;
Floods;
Gene Expression Regulation, Plant;
Glucosides;
biosynthesis;
Luteolin;
biosynthesis;
Phylogeny;
Plant Proteins;
genetics;
Stress, Physiological
- From:
China Journal of Chinese Materia Medica
2018;43(1):52-57
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the effects of the expression of flavonoid 3' hydroxylase gene ( and active ingredients in under flooding stress, we cloned F3'H from Hangju (temporarily named ) and conducted bioinformatics analysis. During the flower bud differentiation stage, we flooded the and then used the Real-time PCR to detect the relative expression of ; Finally, active ingredients of the inflorescence were measured by HPLC.The sequencing results showed that 1 562 bp sequence was acquired with the largest open reading frame of 1 527 bp, which encoded 508 amino acids. The phylogenetic tree found that was highly homologous to other species of Compositae. Real-time PCR results showed that had a significant response to flooding stress and had the highest expression level after flooding for 24 h, which was about 9 times as that of the control group. The results of HPLC showed that luteolin and luteoloside, the downstream products catalyzed by the F3'H, were significantly higher than those in the control group. It was also found that the contents of chlorogenic acid and 3,5- acid were also significantly higher than those of the control group. Therefore, regulates the synthesis of downstream products by regulating the expression of in the flavonoid synthesis pathway under flooding stress, thereby responding to flooding stress. The flooding stress during flower bud differentiation can significantly enhance the accumulation of active ingredients.
