Expression of saponin biosynthesis related genes in different tissues of .
10.19540/j.cnki.cjcmm.20171030.006
- Author:
Kang-Yu WANG
1
;
Wei-Can LIU
1
;
Mei-Ping ZHANG
1
;
Ming-Zhu ZHAO
1
;
Yan-Fang WANG
2
;
Li LI
1
;
Chun-Yu SUN
1
;
Ke-Xin HU
1
;
Yue-Yi CONG
1
;
Yi WANG
1
Author Information
1. College of Life Science, Jilin Agricultural University, Changchun 130118, China.
2. Research Center for Ginseng Genetic Resources Development and Utilization, Changchun 130118, China.
- Publication Type:Journal Article
- Keywords:
Panax quinquefolius;
correlation;
gene expression;
ginsenoside biosynthesis pathway;
real-time quantitative PCR
- MeSH:
Biosynthetic Pathways;
Chromatography, High Pressure Liquid;
Ginsenosides;
biosynthesis;
genetics;
Panax;
genetics;
Plant Roots;
Saponins;
biosynthesis;
genetics
- From:
China Journal of Chinese Materia Medica
2018;43(1):65-71
- CountryChina
- Language:Chinese
-
Abstract:
The relationship between saponin content of in different parts of the organization and expression of ginsenoside biosynthesis related gene was obtained by the correlation analysis between saponin content and gene expression. The 14 tissue parts of were studied, six saponins in Samples (ginsenoside Rg₁, Re, Rb₁, Rc, Rb₂ and Rd), group saponins and total saponins were determined by high performance liquid chromatography and vanillin-sulfuric acid colorimetric method. Simultaneously, the expression levels of 7 ginsenoside biosynthesis related genes ( and ) in different tissues of were determined by Real-time fluorescence quantitative PCR. Although 7 kinds of ginsenoside biosynthesis related enzyme gene in the involved in ginsenoside synthesis, the expression of and P450 genes had no significant effect on the content of monosodium saponins, grouping saponins and total saponins, and had significant or extremely significant on the contents of single saponins Re, Rg1, Rb1, Rd, group saponin PPD and PPT, total saponin TMS and total saponin TS (<0.05 or <0.01). The biosynthesis of partial saponins, grouping saponins and total saponins in was affected by the interaction of multiple enzyme genes in the saponin synthesis pathway, the content of saponins in different tissues of was determined by the differences in the expression of key enzymes in the biosynthetic pathway. Therefore, this study further clarified that and was the key enzyme to control the synthesis of saponins in by correlation analysis, the biosynthesis of ginsenosides in was regulated by these five kind of enzymes in cluster co-expression of interaction mode.
