Research on preparation process of andrographolide-glycyrrhizic acid polymeric micelles.
10.19540/j.cnki.cjcmm.20171107.001
- Author:
Ying LI
1
;
Li-Fang WANG
1
;
Jin-Ling WANG
1
;
Peng-Fei TU
1
Author Information
1. Modern Research Center for Traditional Chinese Medicine， School of Chinese Materia Medica, Beijing University of Chinese Medicine， Beijing 100029， China.
- Publication Type:Journal Article
- Keywords:
andrographolide;
film-thin dispersion method;
glycyrrhizic acid;
loading efficiency;
micelles;
synergistic anti-tumor effects
- MeSH:
Antineoplastic Agents;
pharmacology;
Diterpenes;
pharmacology;
Drug Carriers;
chemistry;
Glycyrrhizic Acid;
chemistry;
Micelles;
Particle Size;
Polymers
- From:
China Journal of Chinese Materia Medica
2018;43(1):79-85
- CountryChina
- Language:Chinese
-
Abstract:
This study aimed to prepare andrographolide (AP)-loaded glycyrrhizic acid (GA) micelles (AP-GA)-PMs to enhance the solubility and anti-tumor effect of andrographolide. Firstly, andrographolide (AP) was used as the model drug and glycyrrhizic acid (GA) as carriers to prepare (AP-GA)-PMs. Then the preparation methods and the ratios of drug and carrier were screened and optimized based on particle size, encapsulation efficiency (EE) and loading capacity of micelles. Finally, the pharmaceutical characters and the inhibition rate on HepG2 cells were evaluated on the (AP-GA)-PMs prepared by optimal process. The results showed that the prepared micelles under the optimal process had a nanosize of (127.11±1.38) nm, zeta potential of (-24.01±0.55) mV, the entrapment efficiency rate of (92.01±4.02)% , the drug loading rate of (51.44±1.24)% and high storage stability at 4 °C in 30 d, with slow but highly stable release. Moreover, (AP-GA)-PMs with the IC₅₀ value of 19.25 mg·L⁻¹ had a more synergistic and better anti-tumor effect in comparison with AP (IC₅₀=122.40 mg·L⁻¹) on HepG2 cells (P<0.01). In conclusion, the (AP-GA)-PMs prepared with glycyrrhizic acid as a carrier had a small particle size, large drug loading capacity, and high stability, and could significantly improve the anti-tumor effects of AP.
