Preliminary Study on the Effect of Adipocytes on the Biological Behaviors of
Lung Adenocarcinoma A549 Cells in Tumor Microenvironment.
10.3779/j.issn.1009-3419.2018.05.01
- Author:
Hang ZHANG
1
;
Jingjing LI
1
;
Yanan CAO
1
;
Xiang DONG
1
;
Cong GAO
1
;
Fanfan LI
1
Author Information
1. Department of Oncology, the Second Affiliated Hospital of Anhui Medical University, Hefei 230601, China.
- Publication Type:Journal Article
- Keywords:
Adipocyte;
Cell proliferation;
Invasion;
Lung neoplasms;
Migration
- MeSH:
A549 Cells;
Adenocarcinoma;
metabolism;
pathology;
physiopathology;
Adenocarcinoma of Lung;
Adipocytes;
cytology;
metabolism;
Animals;
Cell Movement;
Cell Proliferation;
Humans;
Lung Neoplasms;
metabolism;
pathology;
physiopathology;
Mice;
NIH 3T3 Cells;
Triglycerides;
metabolism;
Tumor Microenvironment
- From:
Chinese Journal of Lung Cancer
2018;21(5):351-357
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Adipocytes in the tumor microenvironment may provide the metabolic fuel or signal transduction through media and other means to promote a variety of malignant proliferation and invasion, of tumor cells, but their role in lung cancer progression is still unclear. The purpose of this study was to investigate the effect of adipocytes on lung cancer cell biology.
METHODS:3T3-L1 pre-adipocytes were induced into mature adipocytes. The cell morphology was observed by microscopy and Oil Red O staining. MTT assay, colony formation assay, wound-healing and Transwell methods were used to detect lung cancer cell proliferation, migration and invasion ability. The content of triglyceride in cells was determined by colorimetry.
RESULTS:The morphology of lung adenocarcinoma A549 cells became more slender after co-culture with mature adipocytes, and the proliferation and cloning ability were significantly enhanced (P<0.05). In addition, mature adipocytes can also promote the migration ability (P<0.05), invasion ability (P<0.01) and accumulation of intracellular lipid (P<0.05) of A549 cells.
CONCLUSIONS:These findings suggested that adipocytes in tumor microenvironment can promote the proliferation, migration and invasion of lung adenocarcinoma A549 cells, which may be related to lipid metabolism.
