Role of PD 0332991 on the Proliferation and Apoptosis of Vascular Endothelial Cells.
10.3779/j.issn.1009-3419.2018.05.04
- Author:
Chenlong ZHAO
1
;
Minghui LIU
1
;
Yongwen LI
2
;
Hongbing ZHANG
1
;
Ying LI
2
;
Hao GONG
1
;
Yin YUAN
1
;
Weiting LI
1
;
Hongyu LIU
2
;
Jun CHEN
1
Author Information
1. Department of Lung Cancer Surgery, Tianjin Medical University General Hospital, Tianjin 300052, China.
2. Tianjin Key Laboratory of Lung Cancer Metastasis and Tumor Microenvironment, Tianjin Lung Cancer Institute, Tianjin Medical University General Hospital, Tianjin 300052, China.
- Publication Type:Journal Article
- Keywords:
Lung cancer;
PD 0332991;
Tumor angiogenesis
- MeSH:
Angiogenesis Inhibitors;
pharmacology;
Animals;
Apoptosis;
drug effects;
Cell Line, Tumor;
Cell Proliferation;
drug effects;
Cell Survival;
drug effects;
Cyclin-Dependent Kinase 4;
genetics;
metabolism;
Cyclin-Dependent Kinase 6;
genetics;
metabolism;
Endothelial Cells;
cytology;
drug effects;
metabolism;
Humans;
Lung Neoplasms;
drug therapy;
genetics;
metabolism;
physiopathology;
Mice;
Piperazines;
pharmacology;
Pyridines;
pharmacology
- From:
Chinese Journal of Lung Cancer
2018;21(5):375-382
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Angiogenesis is an important process in the development of tumor. PD 0332991, a cell cycle inhibitor, can specifically inhibit CD4/6 phosphorylation and cell cycle progression. In xeongraft mice models, PD 0332991 treated mice had significantly decreased angiogenesis and vascular density compared with the control group, but the mechanism remains unknown. The purpose of this study is to investigate the role and molecular mechanism of PD 0332991 on vascular endothelial cells.
METHODS:EA.hy926 cells, a kind of vascular endothelial cell, were used as the research model. The effects of PD 0332991 on the activity and proliferation of EA.hy926 cells were detected by the MTT, EdU assays. Wound-healing assays and transwell assays were used to determine the effects of PD 0332991 on the mobility of EA.hy926. The influence of PD 0332991 on cell cycle and apoptosis of endothelial cells was tested by flow cytometry, and the Western blot was applied to observe the expression of cell cycle related proteins in EA.hy926 cells treated by PD 0332991.
RESULTS:PD 0332991 significantly inhibited the proliferation and mobility of EA.hy926 cells, caused cell cycle arrest and apoptosis. At the same time, PD 0332991 inhibited the expression of CDK4/6 and phosphorylation of Rb, and thus inhibited the cell cycle progression of EA.hy926 cells.
CONCLUSIONS:PD 0332991 can inhibit the proliferation and activity of endothelial cells and induces apoptosis.
