Optimized expression of heparin sulfotransferases and their application in sulfation of animal derived heparin.
- Author:
Zhengxiong ZHOU
1
;
Bingbing WANG
1
;
Ruirui XU
1
;
Qing LI
1
;
Guocheng DU
1
;
Zhen KANG
1
Author Information
- Publication Type:Journal Article
- Keywords: Pichia pastoris; anticoagulant activity; heparin; heterologous expression; sulfotransferase
- MeSH: Animals; Escherichia coli; Heparin; chemistry; Oligosaccharides; chemistry; Pichia; Sulfotransferases; biosynthesis; Swine
- From: Chinese Journal of Biotechnology 2018;34(11):1784-1793
- CountryChina
- Language:Chinese
- Abstract: Heparin is a very important anticoagulant drug. Currently, heparin is mainly extracted from porcine mucosa. However, animal-derived heparin shows low anticoagulant activity due to the low proportion of the anticoagulant active unit, the GlcNS6S-GlcA-GlcNS6S3S-Ido2S-GlcNS6S pentasaccharide. In this study we proposed an enzymatic strategy to sulfate the animal-sourced heparin to increase the proportion of anticoagulant pentasaccharide and the anticoagulant activity. First, three sulfotransferases HS2ST, HS6ST, and HS3ST were expressed tentatively in Escherichia coli and Pichia pastoris. After measuring the sulfotransferase activity, we confirmed P. pastoris GS115 is the better host for sulfotransferases production. Then, the maltose binding protein (MBP) and thioredoxin (TrxA) were fused separately to the N-terminal of sulfotransferases to increase enzyme solubility. As a result, the yields of HS2ST and HS6ST were increased to (839±14) U/L and (792±23) U/L, respectively. Subsequent sulfation of the animal-sourced heparin with the recombinant HS2ST, HS6ST and HS3ST increased the anticoagulant activity from (76±2) IU/mg to (189±17) IU/mg.
