Analysis of histone modification of MtSERK1 during in vitro regeneration in Medicago truncatula.

Wei Dong; Peixiang WU; Xijiang Liu; Tianxue Gao; Ning Yang; Yuguang Song

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Analysis of histone modification of MtSERK1 during in vitro regeneration in Medicago truncatula.

Author: Wei DONG ¹ ; Peixiang WU ¹ ; Xijiang LIU ¹ ; Tianxue GAO ¹ ; Ning YANG ¹ ; Yuguang SONG ¹
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1. College of Life Sciences, Qufu Normal University, Qufu 273165, Shandong, China.
Publication Type:Journal Article
Keywords: Medicago truncatula; MtSERK1; callus; histone modification; in vitro organogenesis
MeSH: Epigenesis, Genetic; Gene Expression Regulation, Plant; Genome, Plant; Histone Code; Medicago truncatula; genetics; growth & development; Protein Kinases; genetics; Regeneration
From: Chinese Journal of Biotechnology 2018;34(11):1831-1839
CountryChina
Language:Chinese
Abstract: Epigenetic modification, especially histone modification, plays an important role in maintaining plant genome stability, regulating gene expression and promoting regeneration in vitro. MtSERK1 is an important marker gene involved in establishing of embryogenic callus during in vitro regeneration of Medicago truncatula. In order to understand the regulation Epigenetic modification, especially histone modification, plays an important role in maintaining plant genome stability, regulating gene expression and promoting regeneration in vitro. MtSERK1 is an important marker gene involved in establishing of embryogenic callus during in vitro regeneration of Medicago truncatula. In order to understand the regulation relationship between dynamic histone modification and MtSERK1s expression during the processes of in vitro organogenesis, the expression of MtSERK1 was analyzed by qRT-PCR, and the modification status of H3K9me2, H3K4me3 and H3K9ac in the promoter region and different regions included in the gene body was analyzed by chromatin immunoprecipitation (ChIP). We found expression activation of MtSERK1 was related to the dynamic changes of histone H3K4me3 and H3K9ac in the 5' and 3' regions. This study will provide important theoretical guidance for understanding of the regulatory mechanism of MtSERK1 and also for establishing efficient genetic transformation system of Medicago truncatula.