Improvements of motor and cognitive functions in the rats with permanent middle cerebral artery occlusion treated with acupuncture and rota-rod training.
10.13703/j.0255-2930.2019.07.018
- Author:
Jian WEI
1
;
Chun-Zhu XIE
2
;
Qing YUAN
1
Author Information
1. Clinical Medical College of Acupuncture, Moxibustion and Rehabilitation, Guangzhou University of CM, Guangzhou 510405, Guangdong Province, China.
2. Mathematical Engineering Academy of Chinese Medicine, Guangzhou University of CM.
- Publication Type:Journal Article
- Keywords:
Jin’s three-needle;
acupuncture;
behavioristics;
middle cerebral artery occlusion (MCAO);
rehabilitation training;
rota-rod training
- MeSH:
Acupuncture Points;
Acupuncture Therapy;
Animals;
Cognition;
Infarction, Middle Cerebral Artery;
Male;
Rats;
Rats, Sprague-Dawley
- From:
Chinese Acupuncture & Moxibustion
2019;39(7):748-754
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the effects on the recovery of the motor and cognitive functions of the rats with permanent middle cerebral artery occlusion (pMCAO) after treated with 's three-needle acupuncture at head acupoints combined with rota-rod training.
METHODS:A total of 38 male SD rats were randomized into 3 groups, named a sham-operation group (11 rats), a model group (13 rats) and a treatment group (14 rats). The electrocoagulation method was adopted to establish the model of pMCAO on the right cerebrum. Starting from the 1st day after successful modeling, acupuncture was applied to the "three points of intelligence", the "three points of temporal area" and the "three points of brain". Additionally, the rota-rod training was used. Acupuncture was given once a day and the training was three times a day. In the sham-operation group and the model group, empty grasp fixation was performed when acupuncture was applied in the treatment group, and there was no intervention at the rest of the time. There was 1 day of interval after consecutive 6 days of intervention. Totally, the intervention was for 3 weeks. After modeling, the brain section was collected from 3 rats of each group on the 1st day and was stained with TTC to observe the condition of cerebral ischemia. From day 1 to 7, the neurological function score was evaluated. The footprint analysis and rota-rod test were performed on day 1, 7, 14 and 21. The Morris water maze test was performed from day 22 to 26.
RESULTS:Compared with the sham-operation group, cerebral ischemia presented obviously, the score of neurological function was increased, the back front distances on the left were increased on day 1, 7 and 14 separately, the revolutions per minute (RPM) of the rota-rod were reduced at each of the above 4 time points, the latency of navigation trial was increased and the movement time percentage in Q3 quadrant of spatial probe trial was reduced in the model group (<0.01, <0.05). Compared with the model group, the area of cerebral ischemia was not obviously different (>0.05), the score of neurological function was reduced on day 6, the back front distance on the left was reduced on day 14, RPM of the rota-rod were increased on day 14 and 21, the latency of navigation trial were reduced from day 23 to 25 and the movement time percentage in Q3 quadrant of spatial probe trial was increased in the treatment group (<0.01, <0.05).
CONCLUSION:'s three-needle acupuncture at head acupoints combined with rota-rod training improve the behavioral performance of pMCAO rats and promote the recovery of motor and cognitive functions.
