Repairing effect of wheat-grain moxibustion on thin-type endometrium in rats.
10.13703/j.0255-2930.2019.09.012
- Author:
Yu-Jiao HU
1
;
Liang-Jun XIA
1
;
Bing YAO
2
;
Li CHEN
2
;
Jie CHENG
1
;
You-Bing XIA
1
Author Information
1. Nanjing University of CM, Nanjing 210029, Jiangsu Province, China.
2. Reproductive Medicine Center, Nanjing General Hospital of Nanjing Military Region.
- Publication Type:Journal Article
- Keywords:
endometrial receptivity;
estradiol;
thin-type endometrium;
wheat-grain moxibustion
- MeSH:
Animals;
Endometrium;
physiology;
Female;
Moxibustion;
Random Allocation;
Rats;
Rats, Sprague-Dawley;
Triticum;
Vascular Endothelial Growth Factor A
- From:
Chinese Acupuncture & Moxibustion
2019;39(9):963-969
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To observe the repairing effects of estrogen and wheat-grain moxibustion on thin-type endometrium in rats and to explore its possible mechanism.
METHODS:Forty healthy SPF-grade adult female SD rats were randomly divided into a normal group, a model group, an estrogen group and a moxibustion group according to random number table method, 10 rats in each group. The model of thin-type endometrium was established during estrous period in all the groups except for the normal group. No intervention was given in the normal group. The intragastric administration of 2 mL of 0.9% sodium chloride solution was applied the next day after modeling in the model group. The intragastric administration of 2 mL of estradiol was given the next day after modeling in the estrogen group. The wheat-grain moxibustion was given at "Guanyuan" (CV 4) and "Shenshu" (BL 23) the next day after modeling in the moxibustion group, 7 moxa cones for each acupoint. The treatment in 3 groups was given once a day. After three estrous cycles, the samples were collected during estrous period; the thickness and morphology of endometrium were observed by HE staining; the expressions of vimentin, keratin and vascular endothelial growth factor (VEGF) in endometrium tissue were detected by immunohistochemistry; the expressions of HOXA10 and LIF in endometrium tissue were detected by Western blot.
RESULTS:The endometrial thickness in the model group was significantly thinner than that in the normal group (<0.01); compared with the model group, the endometrial thickness in the estrogen group and the moxibustion group were increased significantly (<0.05, <0.01); the endometrial thickness in the moxibustion group was insignificantly higher than that in the estrogen group (>0.05). The expressions of keratin, vimentin and VEGF in endometrium in the model group were significantly lower than those in the normal group (<0.01); compared with the model group, the expressions of keratin, vimentin and VEGF in endometrium in the estrogen group and the moxibustion group were significantly increased (<0.01). The expressions of keratin, vimentin and VEGF in the moxibustion group were insignificantly higher than those in the estrogen group (>0.05). The expressions of HOXA10 and LIF in endometrium in the model group were significantly lower than those in the normal group (<0.01); compared with the model group, the expressions of HOXA10 and LIF in endometrium in the estrogen group and moxibustion group were significantly increased (<0.05).
CONCLUSION:The wheat-grain moxibustion could up-regulate the expressions of keratin, vimentin and VEGF in endometrium to improve the endometrial thickness; in addition, it could increase the levels of factors related to endometrial receptivity including HOXA10, LIF, which improves endometrial receptivity and play a repair role.
