Role of TLR4/NF-κB pathway for early change of synovial membrane in knee osteoarthritis rats.
10.3969/j.issn.1003-0034.2019.01.015
- Author:
Xue-Zong WANG
;
Dao-Fang DING
;
Yan XUE
;
Xin-Feng GU
;
Jian PANG
;
Min ZHANG
;
Yu-Xin ZHENG
1
,
2
;
Yue-Long CAO
;
Hong-Sheng ZHAN
Author Information
1. Shi's Center of Orthopaedics and Traumatology, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
2. sg_zyx1728@126.com.
- Publication Type:Journal Article
- Keywords:
Osteoarthritis, knee;
Rats;
Synovial membrane
- MeSH:
Animals;
Male;
NF-kappa B;
Osteoarthritis, Knee;
Rats;
Rats, Sprague-Dawley;
Signal Transduction;
Synovial Membrane;
Toll-Like Receptor 4
- From:
China Journal of Orthopaedics and Traumatology
2019;32(1):68-71
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study role of TLR4/NF-κB pathway for early change of synovial membrane in knee osteoarthritis rats.
METHODS:Eighteen male SD rats weighted (200±20) g were randomly divided into 2 groups, namely control and model group, and 9 in each group. Knee OA model group was established by using modified Hulth method in model group. Control group was not treated. Synovial tissue and serum was extracted at 4 and 21 d after operation. Expression of CD14, TLR4, IL-1β, TNF-α, ADAMTS-4, MMP-13 were detected by real-time PCR respectively. NF-κB p65 protein was detected by Western-blot; serum concentrations of haluronic acid (HA), N-propeptide of type III procollagen(PIIINP) was detected by Elisa.
RESULTS:Expression of CD14, ADAMTS-4, and NF-κB p65 in model group were higher than that of control group at 4 and 21 days after operation, while expression of TLR4, IL-1β, TNF-α and MMP-13 were higher than that of control group at 21 days after operation(<0.01). Concentration of PIIINP and HA in model group were higher than that of control group at 4 days after operation, while there was no significant difference at 21 days after operation.
CONCLUSIONS:NF-κB pathway could mediate occurrence of KOA by early activating and triggeringg synovial increasingly secreting inflammatory secretion CD14, TLR4, IL-1β, TNF-α, ADAMTS-4, MMP-13, PIIINP and HA.
