Application of Intermediate Mesoderm-like Cells in Kidney Regeneration.
10.3881/j.issn.1000-503X.11157
- Author:
Jian Ye ZHANG
1
;
Yong GUAN
1
;
Feng KONG
2
;
Sheng Tian ZHAO
3
Author Information
1. Department of Urology, the Second Hospital of Shandong University,Jinan 250033,China.
2. Department of Central Research Lab,the Second Hospital of Shandong University,Jinan 250033,China.
3. Department of Urology,Shandong Provincial Hospital of Shandong University,Jinan 250012,China.
- Publication Type:Journal Article
- MeSH:
Adipose Tissue;
Animals;
Cell Differentiation;
Cells, Cultured;
Kidney;
growth & development;
Mesoderm;
cytology;
Rats;
Rats, Wistar;
Regeneration;
Stem Cells;
cytology;
Tissue Engineering;
Tissue Scaffolds
- From:
Acta Academiae Medicinae Sinicae
2019;41(3):291-299
- CountryChina
- Language:Chinese
-
Abstract:
Objective To induce adipose-derived stem cells (ADSCs) to differentiate into intermediate mesoderm (IM)-like cells ,with IM-like cells for recellularizing kidney scaffolds,and then to obtain a tissue-engineering kidney with renal structures and functions through co-culture.Methods After inguinal fat pads of Wistar rats were surgically harvested,the primary ADSCs were isolated,induced,and cultured for stem cell identification. ADSCs were inducted to differentiate into IM-like cells by adding glycogen synthase kinase-3 inhibitor (CHIR99021) and fibroblast growth factor 9 (FGF9) at different stages. Seven days later,the IM-like cells were identified. The induced IM-like cells and well-prepared kidney decellularized scaffolds were co-cultured for 10 days to obtain recellularized tissue-engineered kidneys and their differentiation was identified.Results The ADSCs harvested had osteogenic and adipogenic abilities and could express the stem cell surface markers. After 7 days of induction,the positive expressions of odd-skipped related 1 and paired-box 2 were observed in IM-like cells by immunofluorescence technique. After 10 days of co-culture with kidney decellularized scaffolds,the positive expressions of Wilms'tumor 1,GATA-binding protein-3,and E-cadherin were observed by immunofluorescence technique.Conclusion ADSCs can be induced into IM-like cells,and renal cell differentiation can be observed through combining the induced IM-like cells with kidney decellularized scaffolds.
