Whole Genome Sequence Determination and Analysis of Strain CGMCC 12426.
10.3881/j.issn.1000-503X.10370
- Author:
Hui DONG
1
;
Xuan Hao LI
1
;
Jing Lin CHANG
1
;
Xin HE
1
;
Qin Lian HOU
1
;
Wei LONG
1
Author Information
1. Key Laboratory of Tianjin Radiation and Molecular Nuclear Medicine,Institute of Radiation Medicine,CAMS and PUMC,Tianjin 300192,China.
- Publication Type:Journal Article
- MeSH:
Bacillus subtilis;
genetics;
Genome, Bacterial;
Plasmids;
RNA, Ribosomal, 16S;
genetics;
RNA, Ribosomal, 23S;
genetics;
RNA, Ribosomal, 5S;
genetics;
Sequence Analysis, DNA
- From:
Acta Academiae Medicinae Sinicae
2019;41(3):307-314
- CountryChina
- Language:Chinese
-
Abstract:
Objective To describe the microbiological characteristics of ()CGMCC 12426 and determine and analyze its complete genome sequences.Methods strain CGMCC 12426 genomic DNA sequencing was performed on a single molecule real-time sequencing(SMRT)platform and the annotation was completed in the NCBI Prokaryotic Genomic Annotation Pipeline(pGAP).Results The complete genomic sequences of the released CGMCC 12426 consisted of a 4 138 265-bp circular chromosome and a 74 165-bp plasmid,which resulted in the prediction of 4581 genes including 4222 coding sequences,87 tRNAs,and 30 rRNAs(which included 5S rRNA,16S rRNA,and 23S rRNA).Conclusion The genome sequencing provided a basis for further investigations on the genetic background of and on the metabolic and regulatory mechanisms.
