Effects of Vitamin D Receptor on Mucosal Barrier Proteins in Colon Cells under Hypoxic Environment.
10.3881/j.issn.1000-503X.11243
- Author:
Zheng WANG
1
;
Hong YANG
1
;
Meng JIN
1
;
Hui Min ZHANG
1
;
Xuan Fu CHEN
1
;
Mei Xu WU
1
;
Ming Yue GUO
1
;
Chang Zhi HUANG
2
;
Jia Ming QIAN
1
Author Information
1. Department of Gastroenterology,PUMC Hospital,CAMS and PUMC,Beijing 100730,China.
2. State Key Laboratory of Molecular Oncology,Cancer Hospital,CAMS and PUMC,Beijing 100021,China.
- Publication Type:Journal Article
- MeSH:
Antigens, CD;
metabolism;
Cadherins;
metabolism;
Cell Hypoxia;
Cell Line;
Claudin-1;
metabolism;
Colon;
cytology;
Humans;
Occludin;
metabolism;
Receptors, Calcitriol;
metabolism;
Tight Junctions;
Vitamin D;
pharmacology;
Zonula Occludens-1 Protein;
metabolism
- From:
Acta Academiae Medicinae Sinicae
2019;41(4):506-511
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the expressions of mucosal barrier proteins in colon cell line DLD-1 under hypoxic environment and its mechanism. Methods After DLD-1 cells were treated separately with hypoxia(l% O),vitamin D(100 nmol/L),or vitamin D plus hypoxia for 48 hours,the expressions of vitamin D receptor(VDR),tight junction proteins zonula occludens-1(ZO-1),occludin,Claudin-1,and adherent junction protein(E-cadherin)were determined by Western blot.Stable VDR knock-down(Sh-VDR)DLD-1 cell line and control DLD-1 cell line were established by lentivirus package technology and the protein expressions after hypoxia treatment were detected. Results Compared with control group,the expressions of occludin,Claudin-1,and VDR increased significantly after hypoxia treatment(all <0.001).In addition to the protein expressions of occludin,Claudin-1 and VDR,the expressions of ZO-1 and E-cadherin were also obviously higher in vitamin D plus hypoxia group than in single vitamin D treatment group(all <0.001).After hypoxia treatment,Sh-VDR cell line showed significantly decreased expressions of ZO-1(<0.001),occludin(<0.05),Claudin-1(<0.01)and E-cadherin(<0.001)when compared with untreated Sh-VDR cell line. Conclusion VDR acts as a regulator for the expressions of intestinal mucosal barrier proteins under hypoxia environment in DLD-1 colon cell line,indicating that VDR pathway may be another important protective mechanism for gut barrier in low-oxygen environment.
