Effects of moxibustion at 45 ℃ on blood lipoids and serum level of ox-LDL and NO in rats with hyperlipidemia.
10.13703/j.0255-2930.2019.02.018
- Author:
Fang-Fang SU
1
;
Jian-Yun GAO
2
;
Gui-Ying WANG
2
;
Ruo-Yang CHEN
2
;
Yao-Shuai WANG
3
;
Hui-Fang ZHANG
4
Author Information
1. Graduate School of Medical College of South-East University, Nanjing 210009, Jiangsu Province, China.
2. Department of Acupuncture and Moxibustion, Zhongda Hospital of South-East University, Nanjing 210009, Jiangsu Province.
3. the Second Clinical Medical College of Nanjing University of CM.
4. Department of Acupuncture and Moxibustion, the First People's Hospital of Changzhou City.
- Publication Type:Journal Article
- Keywords:
anti-oxidant stress;
hyperlipidemia;
moxibustion;
nitric oxide (NO);
oxidized low density lipoprotein (ox-LDL);
regulating effects on blood lipid;
vascular endothelium
- MeSH:
Animals;
Hyperlipidemias;
Lipoproteins, LDL;
Moxibustion;
Nitric Oxide;
Rats;
Rats, Sprague-Dawley
- From:
Chinese Acupuncture & Moxibustion
2019;39(2):180-184
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To observe the effects of moxibustion at different temperatures (38 ℃ and 45 ℃) on blood lipoids and serum level of oxidized low density lipoprotein (ox-LDL) and nitric oxide (NO) in rats with hyperlipidemia, and to explore the correlation between regulating blood fat and anti-oxidative stress and protection of vascular endothelium of moxibustion at 45 ℃.
METHODS:According to random number table, 60 SD rats were randomly divided into a normal group, a model group, a moxibustion at 38 ℃ group and a moxibustion at 45 ℃ group, 15 rats in each group. The rats in the normal group received no treatment; the rats in the remaining three groups were fed with high-fat diet for 8 weeks to prepare rat models of hyperlipidemia. After successful modeling, the rats in the model group received no treatment; the rats in the moxibustion at 38 ℃ group and moxibustion at 45 ℃ group were treated with moxibustion at "Shenque" (CV 8) and "Zusanli" (ST 36), and the temperature was controlled at (38±1) ℃ and (45±1) ℃, respectively. The moxibustion was given for 10 min at each acupoint, once every two days, and totally 4-week treatment was given. After treatment, the levels of total cholesterol (TC), triglyceride (TG), high-density lipoprotein cholesterol (HDL-C) and low-density lipoprotein cholesterol (LDL-C) were measured by using biochemical colorimetric method; the levels of ox-LDL and NO were measured by using ELISA method.
RESULTS:① Compared with the normal group, the levels of TC, TG and LDL-C were significantly increased in the model group (all <0.01); compared with the model group and moxibustion at 38 ℃ group, the levels of TC, TG and LDL-C were significantly decreased in the moxibustion at 45 ℃ group (<0.01，<0.05); compared with the model group, the levels of TC, TG and LDL-C were insignificantly decreased in the moxibustion at 38 ℃ group (all >0.05). ② Compared with the normal group, the level of ox-LDL was increased but that of NO was decreased in the model group (both <0.01); compared with the model group and moxibustion at 38 ℃ group, the level of ox-LDL was decreased but that of NO was increased in the moxibustion at 45 ℃ group (<0.01, <0.05); compared with the model group, the level of ox-LDL was decreased but that of NO was increased in the moxibustion at 38 ℃ group (both <0.05).
CONCLUSION:Moxibustion at 45 ℃ has regulating effects on blood lipid in rats with hyperlipidemia, which can regulate blood lipid through various ways, such as anti-oxidative stress and protection of vascular endothelium.
