Transcriptomic microarray profiling of peripheral CD4+ T cells from asthmatic patients.
10.3760/cma.j.issn.1003-9406.2018.06.012
- Author:
Min ZHU
1
;
Min HE
;
Yarong HE
;
Yulin JI
Author Information
1. Department of Respiratory Medicine, West China Hospital of Sichuan University, Chengdu, Sichuan 610041, China. 790754788@qq.com.
- Publication Type:Journal Article
- MeSH:
Antigens, CD;
genetics;
Asthma;
immunology;
CD4-Positive T-Lymphocytes;
cytology;
Case-Control Studies;
Eosinophils;
Gene Expression Profiling;
Humans;
Leukocytes, Mononuclear;
Oligonucleotide Array Sequence Analysis;
Receptors, Granulocyte-Macrophage Colony-Stimulating Factor;
genetics;
Receptors, Immunologic;
genetics;
Transcriptome
- From:
Chinese Journal of Medical Genetics
2018;35(6):828-831
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To identify differentially expressed genes in peripheral blood mononuclear cells between patients with continuous mild-to-moderate asthma and healthy controls using mRNA microarray in order to explore the underlying signaling pathways and clarify the roles of CD4+ T cells in the pathogenesis of asthma.
METHODS:Global transcriptomic profiles of the CD4+ T cells were defined by using Agilent Sure Print G3 Human GE 8×60K microarray. Enrichment pathways were analyzed with Ingenuity Pathway Analysis (IPA) software.
RESULTS:Compared with controls, 805 genes were up-regulated, 192 were down-regulated in asthma patients. Among these, the expression of 38 annotated genes have varied by 4 times or more. Expression of CD300A was inversely proportional to the absolute value of eosinophils (r=-0.89, P=0.02) as well as the proportion of eosinophils (r=-0.94, P=0.004), while CSF1R was inversely proportional to PD20 (r=-0.83, P=0.04) and AQLQ (r=-0.88, P=0.02) by correlation analysis.
CONCLUSION:Numerous pathophysiological pathways may be involved in the pathogenesis of asthma. Above findings have provided a basis for the delineation the pathogenesis of asthma.
