Analysis of chromosomes of embryos derived from translocation carriers during preimplantation genetic diagnosis cycles.
10.3760/cma.j.issn.1003-9406.2018.06.024
- VernacularTitle:PGD周期中染色体易位携带者的胚胎染色体分析
- Author:
Qiuxiang HUANG
1
,
2
;
Chunli LIN
;
Zhibiao CHEN
;
Yun LIU
;
Jian ZENG
;
Juan LIN
;
Zhihong WANG
Author Information
1. Department of Obstetrics and Gynecology, Center of Reproductive Medicine, Fuzong Clinical College of Fujian Medical University, Dongfang Hospital Affiliated to Xiamen University, Fuzhou, Fujian 350025, China. liuyunfj@126.com
2. xiaonvwang@163.com.
- Publication Type:Journal Article
- MeSH:
Chromosomes, Human;
genetics;
Female;
Genetic Carrier Screening;
Humans;
In Situ Hybridization, Fluorescence;
Male;
Maternal Age;
Pregnancy;
Preimplantation Diagnosis;
Translocation, Genetic
- From:
Chinese Journal of Medical Genetics
2018;35(6):875-878
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the effect of chromosomal translocations on the composition of embryonic chromosomes and its mechanism.
METHODS:For 52 couples with one partner carrying a chromosomal translocation, results of next generation sequencing of all embryos derived from 61 cycles were divided into different groups based on the type of translocations, gender of the carrier, and maternal age. Effect of parental chromosomal translocations on the composition of embryonic chromosomes of each group was analyzed.
RESULTS:A significant difference was found between carriers of reciprocal and Robertsonian translocations in terms of proportion of abnormal embryos and structurally normal chromosomes (63.3% vs. 27.5%, and 1.1% vs. 0.3%, respectively). Compared with male carriers, there was an increase in the rate of abnormalities for female carriers (67.2% vs. 58.3% for reciprocal translocations, and 45.5% vs. 13.8% for Robertsonian translocations). The risk for chromosomal abnormality also increased with the maternal age. No significant difference was found in the proportion of abnormal embryos between carriers divided by involvement of acrocentric chromosomes or terminal chromosomal breakpoints.
CONCLUSION:The types of parental translocation, gender of carrier, maternal age, and interchromosomal effect have certain effect on the composition of embryonic chromosomes.
