Molecular cytogenetic characterization of five patients with myeloid leukemia and t(12;22)(p13;q12).
10.3760/cma.j.issn.1003-9406.2019.02.004
- Author:
Haigang SHAO
1
;
Qian YANG
;
Yanlei GONG
;
Shuxiao BAI
;
Jun ZHANG
;
Yong WANG
;
Juan SHEN
;
Chunxiao WU
;
Huiying QIU
;
Suning CHEN
;
Jinlan PAN
Author Information
1. The First Affiliated Hospital of Soochow University, Jiangsu Institute of Hematology, Key Laboratory of Thrombosis and Hemostasis of the Ministry of Health, Suzhou, Jiangsu 215006, China. jinlanpan@126.com.
- Publication Type:Journal Article
- MeSH:
Chromosome Banding;
Chromosomes, Human, Pair 12;
Chromosomes, Human, Pair 22;
Cytogenetics;
Humans;
In Situ Hybridization, Fluorescence;
Leukemia, Myeloid;
genetics;
Oncogene Proteins, Fusion;
Translocation, Genetic
- From:
Chinese Journal of Medical Genetics
2019;36(2):112-115
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the clinical and laboratory characteristics of 5 patients with myeloid leukemia and t(12;22)(p13;q12).
METHODS:Bone marrow cells were cultured for 24 h and analyzed by standard R-banding. Rearrangement of the MN1 gene was detected by fluorescence in situ hybridization (FISH) using dual color break-apart MN1 probes. MN1-ETV6 and ETV6-MN1 fusion genes were detected by reverse transcription polymerase chain reaction (RT-PCR). And the products were subjected to direct sequencing.
RESULTS:Among the 5 patients, 2 had AML-M0, 2 had AML-M4, and 1 had CMML at the initial diagnosis. t(12;22)(p13;q12) was the primary abnormality among all patients. Rearrangements of MN1 gene were detected by FISH in all patients. MN1-ETV6 and ETV6-MN1 fusion genes were detected respectively in 4 and 3 patients.
CONCLUSION:t(12;22)(p13;q12) is a rare but recurrent chromosomal abnormality in myeloid leukemia, and is related to poor prognosis. allo-SCT is valuable for patients with t(12;22)(p13;q12).
