Optimization of chromosome flaking technique for mesenchymal stem cells.
10.3760/cma.j.issn.1003-9406.2019.02.012
- Author:
Maosheng CHEN
1
;
Chuang LI
;
Dehua CHENG
;
Yueqiu TAN
;
Hua MEI
;
Siqi WANG
;
Xiang LI
;
Lamei CHENG
Author Information
1. Institute of Reproductive and Stem cell Engineering, School of Basic Medicine, Central South University, Changsha, Hunan 410013, China. mmlamei@163.com.
- Publication Type:Journal Article
- MeSH:
Cell Culture Techniques;
Cell Differentiation;
Cells, Cultured;
Chromosome Disorders;
Chromosomes, Human;
Cytogenetics;
Humans;
Mesenchymal Stem Cells
- From:
Chinese Journal of Medical Genetics
2019;36(2):143-146
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To optimize the condition for chromosome flaking of mesenchymal stem cells to ensure the cytogenetic quality control of expanding production and clinical application.
METHODS:Chromosomal flaking methods were optimized from current chromosome preparation techniques from the aspects of MSCs cell culture concentration, colchicine treatment time and low permeability time.
RESULTS:By repeated pre-experiments, the optimal MSCS chromosome flaking condition of MSCs was determined as cell culture concentration of (1-2)× 10 cells per T25 cell culture bottle, and the colchicines processing time was determined as 2 hours and 10 minutes, and the low permeability was 1 hour.
CONCLUSION:The optimized chromosome flaking condition can fulfill the requirement of cytogenetic quality control for MSCs.
