Serological and molecular genetic analysis of a family with B(A) blood type.
10.3760/cma.j.issn.1003-9406.2019.02.018
- VernacularTitle:B(A)血型家系的血清学及分子遗传学研究
- Author:
Wenjing ZHANG
1
;
Shouyong HUN
;
Yuxia WANG
;
Yunlong ZHUANG
Author Information
1. Institute of Hematology, Blood Center of Shandong Province, Jinan, Shandong 250014, China. yunlongzhuang@gmail.com.
- Publication Type:Journal Article
- MeSH:
ABO Blood-Group System;
Alleles;
Exons;
Female;
Genotype;
Humans;
Phenotype
- From:
Chinese Journal of Medical Genetics
2019;36(2):165-167
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To analyze the blood type of a family with B(A) blood type.
METHODS:The serological blood type of the family was determined by routine tube method. Exons 6 and 7 of the ABO gene were amplified by PCR and subjected to Sanger sequencing.
RESULTS:Serological testing of the proband and her elder son showed a discrepancy which was initially identified as B(A) subtype. Her husband and second son were identified as blood type O. Sequencing of the proband and her elder son has identified an O allele and a 640A>G mutation compared with the B gene. Her husband and second son possessed the same genotype of O/O.
CONCLUSION:The 640A>G mutation of ABO gene probably underlies the B(A) subtype.
