Sequencing of a large off-ladder allele at Penta E locus.
10.3760/cma.j.issn.1003-9406.2019.02.019
- Author:
Ying'ai ZHANG
1
;
Shunlan WANG
;
Xiaohong WEN
;
Shufang ZHANG
Author Information
1. Central Laboratory, Haikou People's Hospital, Haikou, Hainan 570208, China. zhangyingai@hotmail.com.
- Publication Type:Journal Article
- MeSH:
Alleles;
Polymerase Chain Reaction;
Rubiaceae
- From:
Chinese Journal of Medical Genetics
2019;36(2):168-170
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To identify a rare large off ladder (OL) allele at the Penta E locus.
METHODS:Chelex-100 was used to extract DNAs from the blood samples. The PCR fragments were purified, extracted, and subjected to TA cloning and sequencing.
RESULTS:An OL allele was identified by the PowerPlex™ 21 at the Penta E locus, which was postulated as allele 26 based on assigned size. The OL allele was verified as a novel fragment containing 26 full AAAGA repeats.
CONCLUSION:OL alleles and microvariants should be verified by direct sequencing. Typing of OL alleles has significance for both daily work and forensic genetics.
