Adenanthin Induces Differentiation of Acute Promyelocytic Leukemia Cells by Targeting Peroxiredoxin III.
10.19746/j.cnki.issn.1009-2137.2019.04.015
- Author:
Yun-Xi MO
1
;
Dan-Li LIU
1
;
Shun-Li GAO
1
;
Xiao-Cheng YIN
2
Author Information
1. Department of Pediatrics, The First Hospital Affiliated to University of South China, Hengyang 421001, Hunan Province, China.
2. Department of Pediatrics, The First Hospital Affiliated to University of South China, Hengyang 421001, Hunan Province, China,E-mail: xcyin108@sina.com.
- Publication Type:Journal Article
- MeSH:
Cell Differentiation;
Diterpenes, Kaurane;
HL-60 Cells;
Humans;
Leukemia, Promyelocytic, Acute;
Peroxiredoxin III;
Tretinoin
- From:
Journal of Experimental Hematology
2019;27(4):1083-1087
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the differentiation of acute promyelocytic leukemia (APL) cells induced by adenosine targeting Prx III.
METHODS:HL-60 cells were divided into four groups: control group, all-trans retinoic acid (ATRA) group, adenanthin group and ATRA+adenanthin group. Cell morphologic changes were observed under optical microscope. The influence of adenanthin on the differentiation of HL-60 was observed by nitro blue tetrazolium chloride (NBT) test. Cell surface differentiation antigens CD11b expression was measured by flow cytometry. The protein expression of Prx III was detected by immunohistochemical assay.
RESULTS:Adenanthin could induce the differentiation of HL-60 cells; the NBT reduction positive rate in ATRA+adenanthin group was significantly higher than that in ATRA group and adenanthin group (P＜0.05). The percentage of CD11b positive cells in ATRA+adenanthin group (43.62％±1.38％) was higher than that in adenanthin group (28.15％±1.78％), ATRA group (36.72％±1.33％) and control group (7.99％±1.78％) (P＜0. 05). The content of Prx Ⅲ protein in adenanthin group was significantly higher than that in control group and ATRA group (P＜0.05).
CONCLUSION:Adenanthin and ATRA have a synergistic effect on the differentiation and maturation of HL-60 cells, and its mechanism may be related with regulation of Prx III expression.
