Focal adhesion linker proteins expression of fibroblast related to adhesion in response to different transmucosal abutment surfaces.
- Author:
Yeon Hee MOON
1
;
Mi Kyeong YOON
;
Jung Sun MOON
;
Jee Hae KANG
;
Sun Hun KIM
;
Hong Seo YANG
;
Min Seok KIM
Author Information
- Publication Type:Original Article
- Keywords: Transmucosal abutment; Focal adhesion linker proteins; Gingival fibroblast; Adhesion
- MeSH: Cell Survival; Cells, Cultured; Ceramics; Dental Implants; Fibroblasts; Focal Adhesions; Humans; Microscopy, Electron, Scanning; Paxillin; Proteins; Seeds; Titanium; Ultrasonics; Vinculin
- From:The Journal of Advanced Prosthodontics 2013;5(3):341-350
- CountryRepublic of Korea
- Language:English
- Abstract: PURPOSE: To evaluate adherence of human gingival fibroblasts (HGFs) to transmucosal abutment of dental implant with different surface conditions with time and to investigate the roles of focal adhesion linker proteins (FALPs) involved in HGFs adhesion to abutment surfaces. MATERIALS AND METHODS: Morphologies of cultured HGFs on titanium and ceramic discs with different surface were observed by scanning electron microscopy. Biocompatibility and focal adhesion were evaluated by ultrasonic wave application and cell viability assay. FALPs expression levels were assessed by RT-PCR and western blot. RESULTS: There seemed to be little difference in biocompatibility and adhesion strength of HGFs depending on the surface conditions and materials. In all experimental groups, the number of cells remaining on the disc surface after ultrasonic wave application increased more than 2 times at 3 days after seeding compared to 1-day cultured cells and this continued until 7 days of culture. FALPs expression levels, especially of vinculin and paxillin, also increased in 5-day cultured cells compared to 1-day cultured fibroblasts on the disc surface. CONCLUSION: These results might suggest that the strength of adhesion of fibroblasts to transmucosal abutment surfaces increases with time and it seemed to be related to expressions of FALPs.
