Relationship of Peripheral Blood IL-37 Expression with T Lymphocytes Subsets and NK Cells in Patients with Primary Immune Thrombocytopenia.
10.19746/j.cnki.issn.1009-2137.2019.04.034
- Author:
Zhen CHEN
1
;
Wen QU
2
;
Hua-Quan WANG
1
;
Li-Min XING
1
;
Yu-Hong WU
1
;
Zhao-Yun LIU
1
;
Yang ZHANG
1
;
Hui LIU
1
;
Xi-Feng DONG
1
;
Jing-Lian TAO
1
;
Zong-Hong SHAO
3
Author Information
1. Department of Hematology，General Hospital of Tianjin Medical University，Tianjin 300052，China.
2. Department of Hematology，General Hospital of Tianjin Medical University，Tianjin 300052，China,E-mail: quwentj923@sina．com.
3. Department of Hematology，General Hospital of Tianjin Medical University，Tianjin 300052，China,E-mail: shaozonghong@ sina．com.
- Publication Type:Journal Article
- MeSH:
CD8-Positive T-Lymphocytes;
Flow Cytometry;
Humans;
Interleukin-1;
immunology;
Killer Cells, Natural;
Leukocytes, Mononuclear;
Purpura, Thrombocytopenic, Idiopathic;
T-Lymphocyte Subsets
- From:
Journal of Experimental Hematology
2019;27(4):1201-1207
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the correlation of IL-37 with T lymphocytes subsets and NK cells in ITP patients, and to explore its possible mechanisms involved in the pathogenesis of ITP.
METHODS:Forty-five patients with newly diagnosed ITP(newly diagnosed group), 32 patients of complete remission (remission group) and 22 healthy persons(control group) were selected. The serum level of IL-37 in 3 groups was determined by enzyme linked immunosorbent assay (ELISA). The mRNA expression of IL-37, IL-17 and IL-18 in peripheral blood mononuclear cells（PBMNC） in 3 groups was measured by real-time fluorescence quantitative polymerase chain reaction (PCR). The number of IL-18RαCD4 T cells and Tim-3NK cells in the peripheral blood in 3 groups was detected by flow cytometry (FCM).
RESULTS:The serum level of IL-37 in the peripheral blood of ITP patients in the newly diagnosed group was significantly higher than that in the control group and the remission group(P＜0.01) . The expression level of IL-37 in PBMNC of the ITP patients in newly diagnosed group was higher than that in the control group and the remission group(P＜0. 05). The expression level of IL-17 and IL-18 in PBMNC of the ITP patients in newly diagnosed group was higher than that in the control group and the remission group(P＜0. 01); the expression of IL-18Rα in CD4 T cells in newly diagnosed group was significantly higher than that in both the control and the remission group(P＜0.01).The expression of Tim-3 in NK cells in ITP patients was significantly lower than that in the control group (P＜0. 01). In ITP patients, the serum IL-37 level and IL-18RαCD4T cells ratio both negatively correlated with Plt count (r=-0.58, r=-0.48) moreo-ver the serum IL-37 level also negatively correlated with amount of CD4 T cells and NK cells (r=-0.29, r=-0.28), but positively correlated with amount of CD8 T cells (r=0.329).
CONCLUSION:The IL-37 and its receptors may play an immunoregulatory role in CD4 T cells and NK cells, the IL-37 may be a therapeutic target for ITP patients.
