Effects of Bone Marrow Mesenchymal Stem Cells Derived from Patients with Newly Diagnosed Acute Myeloid Leukemia on the Cell Proliferation, Cell Cycle and Immunophenotypes of HL-60 Cells.
10.19746/j.cnki.issn.1009-2137.2019.04.044
- Author:
Hong-Mei NING
1
;
Jun WANG
1
;
Yong-Feng SU
1
;
Chen XU
1
;
Jiang-Wei HU
1
;
Xiao LOU
1
;
Xiu-Sen LI
2
;
Ning MAO
3
;
Hu CHEN
4
Author Information
1. Department of Hematopoietic Stem Cell Transplantation, The Fifth Medical Center of Chinese PLA General Hospital, Beijing 100071,China.
2. Institute of Military Cognition and Brain Sciences, Academy of Military Medical Sciences, Academy of Military Sciences,Beijing 100850, China.
3. Institute of Military Cognition and Brain Sciences, Academy of Military Medical Sciences, Academy of Military Sciences,Beijing 100850, China,E-mail: maoning@nic.bmi.ac.cn.
4. Department of Hematopoietic Stem Cell Transplantation, The Fifth Medical Center of Chinese PLA General Hospital, Beijing 100071,China,E-mail: chenhu217@aliyun.com.
- Publication Type:Journal Article
- MeSH:
Bone Marrow Cells;
Cell Cycle;
Cell Proliferation;
HL-60 Cells;
Humans;
Immunophenotyping;
Leukemia, Myeloid, Acute;
Mesenchymal Stem Cells;
Tumor Microenvironment
- From:
Journal of Experimental Hematology
2019;27(4):1259-1264
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the role of bone marrow microenvironment(niche) in the development of acute myeloid leukemia (AML) and the effect of AML patients-derived MSC on the proliferation, cell cycle and immuno-phenotypes of HL-60 cells.
METHODS:The MSC derived from bone marrow of patients with newly diagnosed AML were isolated and co-cultured with HL-60 cells. The effect of MSC on proliferation of HL-60 cells was detected by using 3H-TdR incorporation method, the cell cycle and immunophenotypes of HL-60 cells were detected by flow cytometry.
RESULTS:The results of 3H-TdR incorporation assay showed that both AML-MSCs and normal MSCs remarkably suppressed the HL-60 cell proliferation in a time- and dose-dependent manner. The results of cell cycle analysis demonstrated that AML MSCs and normal MSCs induced arrest of the HL-60 cells in G/G phase. The results of immunophenotyping revealed that MSCs suppressed the expression of CD11a and CD154 on the surface of HL-60 cells. Moreover, AML MSCs exhibited increased inhibitory effects than that of normal MSCs. However, no remarkable effect of MSCs on CD54 expressions of HL-60 cells was observed in the current study.
CONCLUSION:AML-MSCs possess effects on HL-60 cell proliferation, cell cycle and immunophenotypes similiar to normal MSCs, but exhibited increased suppressive capacity on the expression of CD11a and CD154.
