Bone Marrow Mesenchymal Stem Cells from Newly Diagnosed Acute Myeloid Leukemia Patients Exhibit Enhanced Osteogenic Differentiation Capacity.
10.19746/j.cnki.issn.1009-2137.2019.04.047
- Author:
Hong-Mei NING
1
;
Jun WANG
1
;
Yong-Feng SU
1
;
Chen XU
1
;
Jiang-Wei HU
1
;
Xiao LOU
1
;
Xiu-Sen LI
2
;
Ning MAO
3
;
Hu CHEN
4
Author Information
1. Department of Hematopoietic Stem Cell Transplantation, The Fifth Medical Center of Chinese PLA General Hospital, Beijing 100071,China.
2. Institute of Military Cognition and Brain Sciences, Academy of Military Medical Sciences, Academy of Military Sciences,Beijing 100850, China.
3. Institute of Military Cognition and Brain Sciences, Academy of Military Medical Sciences, Academy of Military Sciences,Beijing 100850, China,E-mail: maoning@nic.bmi.ac.cn.
4. Department of Hematopoietic Stem Cell Transplantation, The Fifth Medical Center of Chinese PLA General Hospital, Beijing 100071,China,E-mail: chenhu217@aliyun.com.
- Publication Type:Journal Article
- MeSH:
Bone Marrow Cells;
Cell Differentiation;
Cells, Cultured;
Humans;
Leukemia, Myeloid, Acute;
Mesenchymal Stem Cells;
Osteogenesis;
Tumor Microenvironment
- From:
Journal of Experimental Hematology
2019;27(4):1277-1286
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the influence of acute myeloid leukemia (AML) microenvironment on mesenchymal stem cells (MSCs).
METHODS:MSCs were isolated from the bone marrow of newly diagnosed AML patients (AML-MSCs) and were cultured. The morphology of MSC was observed by inverted microscopy, the immunophenotypes of MSC were detected by flow cytometry, the proliferation ability of MSC was detected by using MTT method, the multi-differentation ability of MSC was assayed by osteogenic, lipogenic and chrondrogenic induction. The morphologic features, immunophenotypic characteristics, cell proliferation, and multipotential differentiation capability were compared between the MSC derived from normal healthy donors and AML patients.
RESULTS:AML-MSCs presented the morphological features similar to the normal MSCs. In addition, AML-MSCs highly expressed CD29, CD44, CD73, CD105 and HLA-ABC. Meanwhile, they were homogenously negative for CD14，CD31, CD34, CD45, CD80, CD86 and HLA-DR. Further-more, AML-MSCs showed cell proliferation ability similar to normal MSCs. Notably, AML-MSCs exerted increased osteogenic-differentiation capacity as compared with normal MSCs.
CONCLUSION:AML-MSCs possess typical MSC phenotypes but displayed enhanced osteogenic-differentiation capacity.
