Influence of Oridonin on the Icilling Acitivity of NK-92 MI Cells Targeting Cell THP1 and Its Mechanism.
10.19746/j.cnki.issn.1009-2137.2019.05.004
- Author:
Yan-Feng LIU
1
;
Yan JIA
2
;
Peng-Cheng HE
3
;
Mei ZHANG
3
;
Qun HE
4
Author Information
1. Department of Hematology, Xiangya Hospital, Central South University, Changsha 410008, Hunan Province, China,E-mail: liu_xiaoyu2@163.com.
2. Department of Hematology, Xiangya Hospital, Central South University, Changsha 410008, Hunan Province, China.
3. Department of Hematology, The First Affliated Hospital, Xi'an Jiaotong University, Xi'an 710061, Shaanxi Province, China.
4. Department of Hematology, Xiangya Hospital, Central South University, Changsha 410008, Hunan Province, China,E-mail: hequn@163.com.
- Publication Type:Journal Article
- MeSH:
Cell Line, Tumor;
Diterpenes, Kaurane;
pharmacology;
GPI-Linked Proteins;
Histocompatibility Antigens Class I;
Humans
- From:
Journal of Experimental Hematology
2019;27(5):1374-1379
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the influence of oridonin on the killing activity of NK-92 MI cells targeting THP1 and the related mechanism.
METHODS:The killing activity of NK-92 MI to THP1 before and after oridonin treatment was detected by LDH release assay; the expression of natural killer cell ligands activating receptor D (NKG2D, including MICA, MICB, ULBP1, ULBP2 and ULBP3) was detected by real-time quantitative polymerase chain reaction (qRT-PCR) and Western blot respectively; the expression of cytokine TNF-α, TNF-β and IFN-γ in the co-culture supernatant of NK-92 MI cells and THP1 cells were measured by ELISA.
RESULTS:The killing efficiency after oridonin treatment at different effector-target ratio (1:1, 5:1, 10:1) was all significantly up-regulated in comparison with that before oridonin treatment (P＜0.05). QRT-PCR and Western blot showed that the expressions of mRNA and protein levels of MICB, ULBP1, ULBP2 increased to varying degree (P＜0.05), but the expression levels of MICA and ULBP3 were not statistically significant between experimental group and control group (P＞0.05). ELISA results indicated that IFN-γ and TNF-β release were significantly increased after oridonin treatment (P＜0.05), however, the TNF-α release was not statistically different in comparison with control group (P＞0.05).
CONCLUSION:Oridonin can significantly improve killing efficiency of NK-92 MI on THP1, that might be related with up-regulation of MICB, ULBP1 and ULBP2 expression and promotion of IFN-γ and TNF-β release.
