Effect of Metformin on Proliferation Capacity, Apoptosis and Glycolysis in K562 Cells.
10.19746/j.cnki.issn.1009-2137.2019.05.006
- Author:
Hui-Li CHEN
1
;
Ping MA
1
;
Yan-Li CHEN
1
;
Ling SUN
2
;
Ying XING
3
;
Feng WANG
1
;
Fang WANG
1
;
Wei-Jie CAO
1
;
Yu-Min HUANG
1
;
Rong-Hui ZHANG
1
Author Information
1. Department of Hematology, Stem Cell Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450000, Henan Province, China.
2. Department of Hematology, Stem Cell Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450000, Henan Province, China,E-mail: sunling6686@126.com.
3. Department of Hematology, Stem Cell Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450000, Henan Province, China,E-mail: xingy@zzu.edu.cn.
- Publication Type:Journal Article
- MeSH:
Apoptosis;
Cell Proliferation;
Glycolysis;
Humans;
K562 Cells;
Metformin;
pharmacology;
Phosphatidylinositol 3-Kinases
- From:
Journal of Experimental Hematology
2019;27(5):1387-1394
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effect of metformin on the proliferation, apoptosis and energy metabolism of acute myeloid leukemia (AML) K562 cells and the possible mechanism.
METHODS:Different doses (0, 5, 10, 20 and 30 mmol/L) of metformin was added into the K562 cells, which were cultivated for 24 h, 48 h and 72 h. The inverted optical microscope was used to observe the cell growth, CCK 8 was used to detect the cell vitality. The appropriate metformin doses (0, 10, 20 and 30 mmol/L) and the best time (48 h) were selected for subsequent experiments. The flow cytometer with Annexin V-FITC /PI doulde staining was used to detect apoptosis; the glucose detection kit and lactate detection kit were used to detect glucose consumption and lactate production; fluorescence quantitative PCR was used to detect glycolysis-related gene expression, and Western blot was used to detect protein expression.
RESULTS:Metformin inhibited the proliferation of K562 cells in a dose-dependent manner (r=0.92), and the relative survival in the 30 mmol/L group was as low as 19.84% at 72 h. When treated with metformin for 48 h, the apoptosis rates of 0, 10, 20 and 30 mmol/L groups were 5.14%, 12.19%, 26.29% and 35.5%, respectively. Compared with the control group, the glucose consumption and lactate secretion of K562 cells treated with metformin were significantly reduced (P<0.05), and showed a dose-dependent effect(r=0.94,r=0.93,respectively). Metformin inhibited the expression of GLUT1, LDHA, ALDOA, PDK1, and PGK1 genes of K562 cells (P<0.05) showing a dose-dependent manner(r=0.83，r=0.80，r=0.72，r=0.76，r=0.73,respectively). Metformin inhibited the expression of P-Akt, P-S6, GLUT1, LDHA proteins of K562 cells(P<0.05), showing a dose-dependent relationship(r=0.80，r=0.92，r=0.83，r=0.92,respectively).
CONCLUSION:Metformin can inhibit the growth and proliferation of K562 cells and promote the apoptosis of K562 cells by inhibiting glycolysis energy metabolism. PI3K/Akt/mTOR signaling pathway may be one of the molecular mechanisms of metformin on k562 cells.
