Effects of Transcription Factor MZF-1 on Transcriptive Regulation of Acute Monocytic Leukemia-related Gene MLAA-34.
10.19746/j.cnki.issn.1009-2137.2019.05.017
- Author:
Bo LEI
1
;
Wang-Gang ZHANG
2
;
Ai-Li HE
1
;
Yin-Xia CHEN
1
;
Xing-Meim CAO
1
;
Peng-Yu ZHANG
1
;
Wan-Hong ZHAO
1
;
Jian-Li WANG
1
;
Jie LIU
1
;
Xiao-Rong MA
1
;
Yan-Ping ZHANG
1
;
Hui ZHANG
1
Author Information
1. Department of Hematology，The Second Affiliated Hospital，Medical School of Xi'an Jiaotong University，Xi'an 710004，Shaanxi Province，China.
2. Department of Hematology，The Second Affiliated Hospital，Medical School of Xi'an Jiaotong University，Xi'an 710004，Shaanxi Province，China,E-mail: zhangwanggang2003@yahoo.com.
- Publication Type:Journal Article
- MeSH:
Antigens, Neoplasm;
genetics;
Apoptosis Regulatory Proteins;
genetics;
Gene Expression Regulation, Neoplastic;
Genes, Reporter;
Hepatocyte Nuclear Factor 1-alpha;
Humans;
Kruppel-Like Transcription Factors;
metabolism;
Leukemia, Monocytic, Acute;
Promoter Regions, Genetic;
Transcription, Genetic
- From:
Journal of Experimental Hematology
2019;27(5):1463-1468
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the transcriptional regulation of transcription factor MZF-1 on acute monocytic leukemia-related gene MLAA-34.
METHODS:The effect of MZF-1 on the transcriptional activity of MLAA-34 gene promoter was analyzed by luciferase reporter gene detection system and site-directed mutation technique. The EMSA and ChIP assay were used to verify whether MZF-1 directly and specifically binds to the core region of MLAA-34 promoter. The over-expression vector and interference vector of MZF-1 were constructed to transfect U937 cells, and RT-PCR and Western blot were used to detect the transcription and expression changes of MLAA-34 gene.
RESULTS:The transcription factor MZF-1 had a regulatory effect on MLAA-34 gene expression, and the relative luciferase activity was decreased after MZF-1 binding point mutation (P<0.01). EMSA and ChIP experiments demonstrated that MZF-1 could directly bind to MLAA-34 promoter and play a regulatory role. In the over-expression test, the increase of MZF-1 could up-regulate the expression of MLAA-34 (P<0.05). In the interference test, the decrease of MZF-1 could down-regulate the expression of MLAA-34 (P<0.05).
CONCLUSION:Transcription factor MZF-1 can bind to the transcriptional regulatory region on the promoter of MLAA-34 gene and promote the transcription of MLAA-34 gene in acute monocytic leukemia.
