Effect of Asparaginase on Diffuse Large B-Cell Lymphoma Cell Apoptosis and Its Related Mechanism.
10.19746/j.cnki.issn.1009-2137.2019.05.020
- Author:
Xiang GAO
1
;
Jun JIAO
1
;
Meng-Di LIU
1
;
Yuan-Yuan QIN
1
;
Lin-Yan XU
1
;
Wei SANG
1
;
Kai-Lin XU
2
Author Information
1. Blood Disease Institute of Xuzhou Medical University，Xuzhou 221004，Jiangsu Province, China,Center of Diagnosis and Therapy for Lymphoma， The Affiliated Hospital of Xuzhou Medical University，Xuzhou 221002，Jiangsu Province，China.
2. Blood Disease Institute of Xuzhou Medical University，Xuzhou 221004，Jiangsu Province, China,Center of Diagnosis and Therapy for Lymphoma， The Affiliated Hospital of Xuzhou Medical University，Xuzhou 221002，Jiangsu Province，China,E-mail：lihmd@163.com.
- Publication Type:Journal Article
- MeSH:
Apoptosis;
Asparaginase;
Cell Line, Tumor;
Cell Proliferation;
Humans;
Lymphoma, Large B-Cell, Diffuse
- From:
Journal of Experimental Hematology
2019;27(5):1482-1489
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effects and its potential mechanism of asparaginase on proliferation, cell cycle and apoptosis of diffuse large B-cell lymphoma (DLBCL) cell lines.
METHODS:CCK-8 assay was used to detect the effect of asparaginase on proliferation of DLBCL cell lines. Flow cytometry was used to analyze cell cycle and apoptosis. Western blot was used to analyze apoptosis and its potential mechanism.
RESULTS:Asparaginase obviously inhibited the proliferation of multiple DLBCL cell lines and caused G/G cell arrest. Furtherly, asparaginase inhibited the expression of HIF-1α which related to poor prognosis of patients with DLBCL, up-regulated the expression of DR4 and caspase 8, reduce the expression of c-FLIP. Meanwhile, asparaginase induced the expression of pro-apoptotic protein BAX and inhibited the expression of anti-apoptotic protein MCL-1.
CONCLUSION:Asparaginase can inhibit the proliferation of DLBCL cell lines, cause the arrest of cells in G/G and induce apoptosis via the endogenous and exogenous apoptotic pathways.
