Effect of ABCG2 on Side Population Cells and Related Pathways in Multiple Myeloma.
10.19746/j.cnki.issn.1009-2137.2019.05.028
- Author:
Lei WANG
1
;
Yan LI
2
Author Information
1. Department of Hematology, The First Affiliated Hospital of China Medical University, Shenyang 110001, Liaoning Province, China.
2. Department of Hematology, The First Affiliated Hospital of China Medical University, Shenyang 110001, Liaoning Province, China,E-mail: liyan2@medmail.com.cn.
- Publication Type:Journal Article
- MeSH:
ATP Binding Cassette Transporter, Subfamily G, Member 2;
Cell Line, Tumor;
Humans;
Multiple Myeloma;
Neoplasm Proteins;
PTEN Phosphohydrolase;
Phosphatidylinositol 3-Kinases;
Proto-Oncogene Proteins c-akt;
Side-Population Cells;
Signal Transduction
- From:
Journal of Experimental Hematology
2019;27(5):1548-1555
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the biological characteristics of ABCG2 and its effect on side population cells (SP cells) of multiple myeloma (MM) so as to find the way for reversing drug resistance.
METHODS:The silence of ABCG2 expression was performed throngh interfering the MM cells by using siRNA. then the ratio of SP cell of MM, speed of cell proliferation and sensitivity of cells to chermotherapentic drugs before and after interference were compared, the biological functions of ABCG2 and its role in regulation of SP cells and PTEN/PI3K/AKT signaling pathway was clarified.
RESULTS:siRNA interference could down regulate the expression of ABCG2 at both mRNA and protein level. After siRNA interference, the proliferation of multiple myeloma cells was decreased slightly, the expression of PTEN was increased, the activity of PI3K/AKT pathway was inhibited, and the ratio of SP cells was decreased.
CONCLUSION:In multiple myeloma, down-regulation of expression ABCG2 can negatively regulate the expression of PTEN, the PI3K/AKT signaling pathway is inhibited,the ratio of SP decreased,and the response to drug is increased.
